A membrane glycoprotein isolated from embryonic brain has been shown to be a ligand in the formation of cell-cell bonds. The molecule, called neural cell adhesion molecule (N-CAM), participates in several aspects of neural development, including the formation of plexiform layers, neurite fasciculation and nerve-muscle interactions. Experiments in vivo indicate that N-CAM-mediated adhesion is essential to the orderly assembly of the visual system.

Protein kinase C has a crucial role in signal transduction for a variety of biologically active substances which activate cellular functions and proliferation. When cells are stimulated, protein kinase C is transiently activated by diacylglycerol which is produced in the membrane during the signal-induced turnover of inositol phospholipids. Tumour-promoting phorbol esters, when intercalated into the cell membrane, may substitute for diacylglycerol and permanently activate protein kinase C. The enzyme probably serves as a receptor for the tumour promoters. Further exploration of the roles of this enzyme may provide clues for understanding the mechanism of cell growth and differentiation.

Man shares uniquely few morphological features with either the chimpanzee or the gorilla, whereas there are many features that suggest affinities between man and the orang-utan, to whom the fossil Sivapithecus appears to be closely related. If these are unique features, inclusion of Sivapithecus, man and the orang-utan in a single clade, distinct from that containing the African apes, is justified but contrary to current opinion.

Single-channel recording by the patch-clamp technique has now characterized three kinds of membrane potassium channels activated by intracellular calcium ions in animal cells. These play a crucial part in the regulation of membrane potential and of secretion.

Protein phosphorylation represents an approach, sometimes the only approach available, to study the molecular basis for a wide variety of neurophysiological phenomena. The injection of protein kinases or protein kinase inhibitors into neurones has provided direct evidence that activation of protein kinases has an obligatory role in the mechanisms by which numerous extracellular signals produce specific physiological responses in neurones. A diversity of substrate proteins for the kinases have already been found. In several instances, the identity and functional role of these substrate proteins have been established.

Gene cloning and yeast DNA transformation techniques have greatly enhanced the power of classical yeast genetics. It is now possible to isolate any classically defined gene, to alter the yeast genome at will by replacing normal chromosomal sequences with mutated derivatives produced in vitro, and to create DNA molecules that behave as autonomous replicons or minichromosomes. These unique features of the new yeast genetics have been used to study many problems in eukaryotic molecular biology.

The hallmark of retroviral transforming genes (onc genes) are specific sequences which are unrelated to essential virion genes but are closely related to sequences in normal cells. Viral onc genes probably originated from rare transductions of these cellular sequences by retroviruses without onc genes. Consequently, it has been suggested that retroviral transforming genes are present in normal cells in a latent form. However, recent structural analyses indicate that viral onc genes and cellular genes, which share specific sequences, are not isogenic. They differ from each other in scattered point mutations and in unique coding regions. The cellular genes containing onc-related sequences are expressed in normal cells compatible with a normal function. There is as yet no functional or consistent circumstantial evidence that these cellular genes cause cancer in animals that are not infected by viruses with onc genes. Therefore, it is still uncertain whether the onc-related cellular genes have oncogenic potential beyond their role as progenitors of retroviral onc genes.

In the genome of a germ-line cell, the genetic information for an immunoglobulin polypeptide chain is contained in multiple gene segments scattered along a chromosome. During the development of bone marrow-derived lymphocytes, these gene segments are assembled by recombination which leads to the formation of a complete gene. In addition, mutations are somatically introduced at a high rate into the amino-terminal region. Both somatic recombination and mutation contribute greatly to an increase in the diversity of antibody synthesized by a single organism.

Technological advances in picosecond spectroscopy have permitted the mechanisms of various chemical, physical and biological processes to be elucidated and understood to a greater degree than ever before. By means of picosecond emission, absorption and Raman spectroscopy, one can probe and measure directly the transient intermediates and kinetics of primary events in complex biological processes. A description of two current types of laser systems--solid-state and synchronously pumped dye lasers--and their application to determining the primary events in the biological processes of dissociation of oxy- and carboxymyoglobin, excited-state relaxation of porphyrins and visual transduction, illustrate the power of picosecond spectroscopy.

Calcium channels in excitable membranes are of great importance for many cellular functions. Modulation of these channels by neurotransmitters and drugs regulates calcium influx into the cell and thereby alters the functional state of the cell. Recently it has become possible to measure properties of single calcium channels directly and to obtain evidence on mechanisms of their modulation.

Important constraints on possible molecular mechanisms of general anaesthesia are derived from a quantitative reappraisal of data on the potency of general anaesthetics on whole animals. Despite their popularity, theories that invoke lipids as the prime target do not look at all promising, and available data point much more plausibly to a direct effect on particularly sensitive proteins. Structural changes of proteins on binding general anaesthetics are probably small but may be sufficient to perturb normal function; alternatively, anaesthetics may compete with an endogenous ligand. The phenomenon of pressure reversal of anaesthesia may simply be due to anaesthetics being squeezed away from their target sites.

The sequence of alpha 1-antitrypsin is in keeping with its role as a tissue scavenger of leukocyte elastase. Two abnormal variants commonly present in Europeans cause a deficiency that predisposes them to a progressive loss of lung elasticity. The nature of the reactive centre helps explain why cigarette smoking greatly accelerates the onset and severity of this degenerative process to give the disease emphysema.

We review here the molecular data that bear on and provide a framework for interpreting hominoid relationships. Man is shown to be most closely related to chimpanzees and gorillas among extant hominoids, with the orang-utan more distantly related to them and the gibbons more distantly still. A fossil ape, Sivapithecus meteai, shares several characters with the orang-utan and is thus probably related to it. S. meteai is part of the Middle Miocene Sivapithecus-Ramapithecus species complex, and if this group forms a valid clade then Ramapithecus must also be considered as being more closely related to the orang-utan than to man. The date of divergence of the orang-utan from the African apes and man is suggested by fossil and molecular evidence to be 10 +/- 3 Myr ago.

In light of present progress in the understanding of how messenger RNA is constructed in eukaryotic cells, the levels of gene control are discussed. Transcriptional control, assessed by the rate of synthesis of specific nuclear RNA, is strongly indicated to be the most frequent level of control. Definition of eukaryotic transcription units as simple (encoding one protein) and complex (encoding two or more proteins) affords a framework in which to discuss control at the level of RNA processing for which several examples also are known. Finally, differential stability of cytoplasmic mRNAs and differential translation, both well established, are briefly described.

Active genes are packaged into an altered nucleosome structure forming a chromosomal domain defined by increased sensitivity to nucleases. This structure, reflecting a potential for transcription, contains sites hypersensitive to nuclease digestion adjacent to the coding regions and may also be distinguished by specific non-histone proteins, variant or modified histones or modified DNA. Its formation, by unfolding of a tightly packed chromatin fibre by factors which might affect DNA supercoiling, may be the first step in gene activation.