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1801. [The cytogenetics of hemopoietic dysplasias].1802. [Expression of oncogenes in transplantable tumors and rodent cell lines].
作者: Iu I Kudriavets.;N P Kiseleva.;A A Asanova.;V G Pinchuk.
来源: Biull Eksp Biol Med. 1988年106卷7期86-8页
The expression of 8 oncogenes in transplanted rodent tumours and cell lines was tested. In 6 cases the synthesis of myc-, fos-, ras-oncogene RNA was observed. The transcription of these oncogenes was observed nonspecifically in tumours of different histological types. No difference in the set of the oncogenes expressed and the size of their transcripts was noticed between transplanted tumours and the cell lines obtained from them. The expression of myb-, sis-, Blym-, erb-B- and abl-was not observed in tested cells.
1803. [Circulating E receptor in the peripheral blood of patients with lung cancer].
作者: L A Sleptsova.;A A Red'ko.;O A Kurtenkov.;A M Malygin.;F Kh Kutushev.
来源: Vestn Khir Im I I Grek. 1988年141卷7期72-3页 1804. [Chromosome changes and human malignant tumors].
The data from home and foreign literature on chromosome changes in human tumours of different genesis and localization are analyzed. The role and significance of changes in the chromosome structure in malignant cells are discussed.
1805. [The genetics of breast cancer, population and familial research and segregation analysis].
The data on clinico-genealogy studies of 1046 probands with breast cancer and their relatives are presented. The nature of inheritance corresponded to the Mendelian model. As to other families, there is no strong evidence for the monogene model both with complete and incomplete penetrance of mutant homo- and heterozygotes. Penetrance of homozygotes was 7.9-30.5%, this being 2.0-7.3% for heterozygotes. The conclusion is drawn that it is necessary to consider the regularities of inheritance of breast cancer in the light of the multifactorial model.
1806. [Karyotypic characteristics of a number of clonal lines of an interspecific mouse-mink hybridoma].
Using G-banding method, a study was made of the karyotypes of mouse myeloma cell line P3-X63-Ag8.653 and some cell hybrid lines originated from a fusion of mink immunized spleen cells and this myeloma. Normal chromosomes 6, 12 and X were not detected in either examined cell of the parental myeloma. The mink chromosomes are preferentially lost from cells of the hybrid lines. These lines varied significantly from cell to cell and from each other in the retention of some mink chromosomes. The karyological study of the hybrid lines revealed some cytogenetical specificities of mouse chromosome composition that were general for cells of hybrid lines, but atypical for myeloma parental cells. This data suggest the cells of myeloma parental line to take part in the processes of somatic hybridization and/or selection for mink immunoglobulin production nonrandomly.
1807. [Viral myc oncogene in the genome of transgenic mice and their progeny].
The fused gag-v-myc oncogene was microinjected into fertilized mouse eggs, which were then implanted into foster mothers. Approximately 26% of the offsprings from injected eggs carried v-myc sequences. 26 of 32 progeny animals were found to be transgenic and some progeny containing the amplified oncogene (about 40 copies per genome). In one F0 and one F1 mice 1.5-2 months after birth the development of tumors was observed: rhabdomyosarcoma and sebaceous carcinoma. In both the cases the tumors were highly differentiated. Because spontaneous tumors of these types are seldom observed in common lines of mice it seems probable that the tumors observed in this study may be associated with the presence of oncogene v-myc.
1808. [Assessment of the risk of recurrence isolated cases of dominantly inherited diseases with incomplete penetrance and age-related dependence].
A modification of the method for risk estimation in isolated cases of autosomal dominant disorders with reduced penetrance is presented. It is based on the Bayesian theorem and considers such parameters as fitness, age-specific expressivity of the gene and the effect of parental age on mutation rate. The definite expression of the risk estimation is proposed. Using hereditary polyposis as an example, possible risks are proposed. The table of risks, depending on the parental age, is given.
1809. [Radiation-induced, irreparable, hereditary changes in cells promoting their neoplastic transformation].
In experiments with model plant tumors (Kalanchoe-ti plasmid Agrobact. tumefaciens C-58D) it was shown that exposure of the recipient plant to low-level gamma-radiation of 2 Gy induced changes in cells that were not repaired over two months promoting tumoral transformations in them. Those changes were shown to persist in the offspring of the exposed somatic cells.
1810. [Cellular oncogene expression in human tumors transplantable to athymic mice].
Expression of 3 cellular oncogenes among 7 ones under investigation is identified in the majority of 20 strains of human tumors, passaged in nude mice without significant specificity as far as the type of the tumor is concerned. The levels of the expression of these 3 oncogenes (c-myc, c-fos, c-ras) were higher than the ones in primary human tumors except for the human melanoma Mel-2 strain, where the expression of c-myb oncogene was identified. All the rest oncogenes (c-mos, B-lym, c-sis, c-myb) showed no expression in human tumors of the examined strains.
1811. [Familial multiple myeloma].1812. [Outlook for the genetic engineering approach in radiobiology].
Present evidence on the use of genetic engineering methods in studying the molecular mechanism of radiation damage and repair of DNA, as well as radiation mutagenesis and carcinogenesis has been summarized. The new approach to radiobiological research has proved to be extremely fruitful. Some previously unknown types of structural disorders in DNA molecule have been discovered, some repair genes isolated and their primary structure established, some aspects of radiation mutagenesis elucidated, and research into deciphering the molecular bases of neoplastic transformations of exposed cells are being successfully investigated. The authors discuss the perspectives of using genetic engineering methods in radiobiology.
1813. [Model systems of carcinogenesis in vitro: the interaction of the ras oncogene with immortalized cells].
Gene transfer experiments have shown that ras effector functions are sufficient to transform cells from a variety of established lines (e. g., mouse NIH3T3 cells). In contrast, primary cells and early passage rodent cells can be transformed by ras oncogenes only at low frequencies, unless cotransfected with collaborating genes such as adenovirus early region IA (EIA) or myc retroviral oncogene homologue. Primary rat embryo fibroblasts (REF) were chosen as a model for the analysis of multistep cellular transformation. Transfection of REF, immortalized by early region of simian adenovirus SA7 with c-Ha-ras oncogene cannot induce their morphological transformation. This phenomenon is observed only after second transfection with the same oncogene. These different cell lines can be used for further analysis of the mechanisms of carcinogenesis.
1814. [Regulation of the transcription of the tyrosine aminotransferase gene by glucocorticoids in Morris hepatoma 7777 and 8994 cells].
Kinetics of tyrosine aminotransferase (TAT) mRNA synthesis in Morris rat hepatoma cell lines 7777 and 8994 after dexamethasone treatment (10(-7) M) was studied by molecular hybridization of the RNA with cloned fragments of TAT gene from rat liver cells. It was demonstrated that initiation of TAT gene transcription increased 20 minutes after glucocorticoid treatment. The level of TAT mRNA was not induced by dexamethasone in rat hepatoma cell line 8994. Actinomycin D prevented the deinduction of TAT by stabilization of TAT mRNA.
1815. [Transfection of a plasmid with a retrovirus promoter: distribution of sequences in the genome of induced tumors].
The structure of the integration site of plasmid with LTR of Rous sarcoma virus (pLTR1,5) in the genome of nude mice tumors, induced as a result of N1H3T3 cells' implantation, cotransfected by pLTR1,5 with the DNA of malignant human glioma cells, carrying amplified c-Ha-ras genome, has been studied. The restriction map of the investigated region of the cell genome was obtained. Molecular cloning of the integrated plasmid and adjacent cell sequences has been carried out. It was shown that the exogenic vector in the DNA of the tumor cells is included in BamHI structure of the repeat of mice genome.
1816. [Tumors of the brain and spinal cord in 2 sisters].1817. [Topological DNA heterogeneity in the nucleoids of human leukocytes].1818. [Familial white sponge nevus of the mucosa].1819. [Absorption cytophotometry of DNA in the diagnosis of cutaneous lymphoma of slow neoplastic development].
作者: I M Raznatovskiĭ.;V V Iastrebov.;M S Bogdanova.;G V Selivanova.;Iu M Rozanov.
来源: Vestn Dermatol Venerol. 1988年2期19-23页 1820. [Polycystic disease in children].
作者: V M Deliagin.;G A Zinov'eva.;I A Narycheva.;E V Frolkova.;E M Deliagina.
来源: Pediatriia. 1988年2期90-7页 |