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1661. [The mitochondrial theory of carcinogenesis].
This is a brief review of different ideas on the mechanism of cell malignization united by the mitochondrial conception of carcinogenesis. According to this conception, primary trigger factors of carcinogenesis (free radicals, carcinogens, heat fluctuations) induce damage to mitochondrial DNA and membranes that results in the rearrangement of cell energy metabolism to glycolytic type and disturbance of mitochondrial structure and reproduction. The injured part of mitochondrial DNA incorporating into nuclear DNA induces activation of oncogenes, appearance of oncoproteins and malignization of cells.
1662. [The role of the constitutional features and rearrangement of the H-ras 1 oncogene in the development of human lung cancer].
Length polymorphism of restriction fragments of oncogene HRASI was studied in 52 patients with lung cancer as compared with corresponding normal tissues and leukocytes of these patients and of healthy volunteers. Enhanced frequency of one of main alleles of HRASI A4 was found to correlate with development of the disease aggressive symptoms. Alterations in the HRASI locus of tumoral DNA appear to correlate distinctly with the elevate frequency of the allele (P less than 0.01). Relationship between the allele A4 and active metastases spreading in lung cancer of the III-IV stages as well as specific rearrangements as a result of which allele A4 maintained unaltered or even amplified in carcinomas enabled to suggest that the allele A4 of HRASI oncogene serves as an endogenous risk factor in impairment with non-small cellular lung cancer in addition to typical exogenous factors such as smoking.
1663. [Effect of flanking regions on the expression of the human interleukin-2 chromosomal gene in a murine myeloma cell line].
作者: N N Anikeeva.;T V Vinogradova.;E S Dement'eva.;A R Ibragimov.
来源: Bioorg Khim. 1991年17卷9期1223-8页
A human interleukin-2 (IL-2) gene was isolated from genomic DNA library. The isolated gene with 5'- and 3'-flanking sequences of various lengths was inserted into plasmids derived from the retroviral vector pPSneo. The recombinant plasmids were transfected into myeloma X63Ag8-653 cells. The transfected cells, harbouring the IL-2 gene with the shortened (to position -165) or totally deleted 5'-flanking sequence, constitutively expressed biologically active IL-2. Deletion of 3'-flanking region on did not affect the IL-2 expression.
1664. [Clinical course of the disease in familial and non-familial forms of serous cystadenocarcinoma of the ovary].
作者: L V Akulenko.;S O Nikogosian.;K I Zhordaniia.;V P Kozachenko.
来源: Akush Ginekol (Mosk). 1991年9期58-60页
A retrospective analysis of the case histories of patients with familial and nonfamilial ovarian carcinomas has shown that patients with the familial condition develop a higher resistance of the body to tumor dissemination and their survival rate is better. This conclusion may be of interest for those who research carcinogenesis mechanisms.
1665. [Determination of the role of DNA cytosine methylation in human genetic individuality].
作者: L B Novikov.;S N Fedorov.;O S Iatsuk.;G I Levanova.;V P Kalinovskiĭ.;K P Khanson.
来源: Biull Eksp Biol Med. 1991年112卷8期197-9页
By the restriction analysis method we established that methylation of the 5'-end cytosine in 5'-m5CC-3' duplexes had individual specific features. This genetic peculiarity did not change even in DNA from human stomach carcinomas.
1666. [Plasma cell myeloma. I. A new concept of disease biology].1667. [The genetic polymorphism of the blood proteins in RID-positive reacting cows].
Cattle herd of Black-and-White, Red and Simmental breeds having positive or negative RID-reaction to leucosis was studied as to the polymorphism of serum blood proteins in three loci: Tf, Am and Cp. One system of polymorphic proteins has been determined as having a higher concentration of homozygotes (Tf) and another one as having a higher concentration of heterozygotes (Am) within one and the same herd among animals with the positive RID-reaction.
1668. [Genotypic differences in the level of N-acetyltransferase activity in mice of inbred lines and their hybrids].
There are "slow" and "rapid" acetylator phenotypes in mice according to N-acetyltransferase activity (E.C. 2.3.1.5) (N-AT). The results of direct, back- and reciprocal crosses of mice lines with rapid acetylation phenotype (C57BL/He) show monogenic autosomic control of acetylation locus (Ac) with rapid allele dominance and sex dependence upon N-AT activity in males. So, genetic polymorphism according to the Ac locus exists in mice as well as in some other animal species and in humans. The results obtained in this study demonstrated the correlation between the N-AT level in mice and their predisposition to malignant tumors as well as dependence of cyclophosphamide action on the N-AT activity upon the genotype of an individual, which suggests a certain role of the Ac locus in tumor development.
1669. [Familial myxomas of the heart].
作者: M A Nechaenko.;L P Cherepenin.;G F Sheremet'eva.;V A Makhovko.;M V Antonov.
来源: Klin Med (Mosk). 1991年69卷6期38-41页
An analysis has been made of the clinical, diagnostic, surgical and morphological aspects of familial cardiac myxomas, potentialities of their intravital diagnosis and prevention. No morphological differences were established between familial and sporadic cases of cardiac myxoma.
1670. [Induction of unstable mutations in Drosophila melanogaster by microinjection of oncogenic virus DNA into polar embryonic plasm. Malignant effect of oncoviral DNA].
We have demonstrated that the ability to induce benign neoplasms We have dominant mode of inheritance in Drosophila melanogaster is the specific feature of oncoviral DNAs. It is supposed that development of this type of neoplasms in Drosophila is connected with the changes in expression of protooncogenes in mutant genome: firstly, the genetic factors directing the development of neoplasms and Drosophila protooncogenes which shared the homology with v-src are localised in the same regions; secondly, there are structural rearrangements in c-src/fps (29A) protooncogene in mutant stocks which display the ability for neoplastic growth.
1671. [Genetic and environmental factors in the families of children with dystrophic bone cysts].
作者: M O Gudushauri.;E F Lordkipanidze.;L T Aladashvili.;I I Taboridze.
来源: Ortop Travmatol Protez. 1991年5期66-9页
On the basis of data of 49 probands' families with dystrophic osseous, cyst, 12 probands' families with giant cell tumor and 55 children from the general population by means of clinical and multidimensional-genetical analysis (cluster, discriminant) have been revealed genetical and environmental factors, contributing to the formation of the osseous cysts in children. There have been developed the directions of the search of the "principal" environmental and genetical factors, determined the qualitative criteria of the factor significance, revealed the structures of the mutual connection of the significant factors and obtained their prognostication value definition.
1672. [Proto-oncogene expression in the liver of male rats of different age].
作者: L B Novikov.;R M Balanskiĭ.;V N Anisimov.;K P Khanson.
来源: Biull Eksp Biol Med. 1991年111卷5期521-2页
The expression of 10 protooncogenes has been quantitatively studied in liver of male rats L10 age of 1, 10.5, 22 and 37 months. It was shown that a number of specific mRNA transcripts and, therefore, the levels of expression of protooncogenes C-MYC, C-FOS, N-MYC, HA-RAS, KI-RAS, SIS, ABL, YES, MOS and MET in rat liver were constant during life span. These data are in accordance with resistance of the rat strain L10 to spontaneous hepatocarcinogenesis.
1673. [Presence of specific mutation of Ha-ras oncogene in skin tumors of mice induced under different experimental conditions].
作者: A S Loktionov.;I G Popovich.;M A Zabezhinskiĭ.;V N Anisimov.
来源: Biull Eksp Biol Med. 1991年111卷4期398-400页
The mutation was revealed with substitution of A for T in the second position of the 61 Ha-ras oncogene codon in the DNA of 31 skin tumours (26 papillomas and 5 carcinomas) and in 23 mice treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA). Part of these mice were F progeny (n-6) and F progeny (n-4) following DMBA administration during pregnancy, another part F progeny (n-5) following ENU action on males prior to mating, the rest mice (n-3) did not undergo additional actions. The mutation under study was revealed only in 3 out of 5 papillomas and in all 5 carcinomas of mice subjected to DMBA administration during pregnancy.
1674. [The DNA-mediated transformation of mouse fibroblasts after radiation damage to the cellular chromatin].
作者: V G Bezlepkin.;L B Ostrovskaia.;T A Bezlepkina.;A I Giaziev.
来源: Radiobiologiia. 1991年31卷2期188-94页
In experiments with NIH3T3 mouse fibroblast the authors showed a dose-dependent (gamma- or UV-radiation) increase in the immortalized fibroblast transformation by purified DNA preparations from Ehrlich ascites tumour cells. The transformant yield was the highest when the transfection started within the first few minutes after irradiation, when radiation lesions occurred in the genome and the system of enzymic DNA repair was activated. The proteolytic activity inhibition by treating the exposed cells with phenylmethanesultonyl fluorine reduced the radiation-induced transformation.
1675. [The behavior of the X chromosomes in intra- and interspecific hybrid cells of murine teratocarcinoma PCC4azal].
作者: N S Zhdanova.;S I Baĭborodin.;A Iu Kerkis.;L D Matiakhina.;O L Serov.
来源: Ontogenez. 1991年22卷2期158-67页
We have demonstrated that X chromosomes are reactivated in hybrids obtained by fusion of mouse PCC4azaI teratocarcinoma cells (XO, 39HPRT-) with splenocytes from mouse females heterozygous in Hprt gene. These hybrids are capable of spontaneous differentiation. We also obtained similar interspecies hybrids of PCC4azaI cells with bone marrow cells of the American mink. The majority of such hybrids remained undifferentiated, however, after long-term cultivation at high cell density they differentiated into epithelial- or fibroblast-like cells similarly to PCC4azaI cells. Two hybrids had the autosomal complement of the mouse and two X chromosomes (mouse and mink); both X chromosomes were active. These X chromosomes were not inactivated during differentiation in vitro.
1676. [The expression of P-glycoprotein in leukemia P388 cells with induced doxorubicin resistance].
作者: V A Sukhanov.;V L D'iakov.;V V Lalaev.;A V Iakh'iaev.;I M Voronkova.;F V Donenko.;N B Borovkova.;L V Moroz.
来源: Biull Eksp Biol Med. 1991年111卷3期290-1页
beta-D-galactose-containing glycoproteins were prepared from cells P-388 leukemia and from P-388 leukemia cells with induced resistance to doxorubicin. It was shown by HPLC method that plasma membranes from resistant cells contain 4-4.5% P-glycoproteins and plasma membranes from sensitive cells contain P-glycoproteins about 10 times lower.
1677. [New antineoplastic antibiotics, new analogs, modifiers of biological reactions and oncogene inhibitors].1678. [Characteristics of pathogenesis of acute leukemia in children].
The main components of acute leukemia pathogenesis have been considered from the current positions: origination of the leukemic clone, characteristics of leukemia tumor histogenesis, mechanisms of proliferation and differentiation of leukemic cells. The main pathogenetic differences of acute leukemia in children and adults have been traced. The importance of these differences for the clinical picture, prognosis and response to chemotherapy has been analyzed.
1679. [Prognostic factors in the development and course of acute lymphoblastic leukemia in children].1680. [The characteristics of the regulation of the cellular proliferation of embryonic rat fibroblasts transformed by E1A+c-Ha-ras oncogenes].
The ability of growth factors (GF) to stimulate proliferation of rat embryo fibroblast (REF) lines immortalized by E 1A oncogene and transformed by complementing E 1A and c-Ha-ras oncogenes (mutant form) was studied. Unlike untreated REF, those immortalized by E 1A oncogene required less serum and GF to proliferate. Proliferation could be stimulated by specific GF alone. Serum appeared to be capable of stimulating proliferation of cells transformed by E 1A + c-Ha-ras oncogenes; however, both GF and proteinases C and A activators failed to exert such effect. Transferrin, usually required by normal cells in late G1-phase, shared the stimulating effect on E 1A+c-Ha-ras-transformed cells. To summarize, E 1A+c-Ha-ras oncogene-transformed REF can grow independently of GF but still require transferrin.
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