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421. [Effect of 5-azadeoxycytidine and retinoic acid on expression of genomic imprinting in parthenogenetic mouse embryos].

作者: L I Penkov.;T K Taseeva.;Ia M Koĭcheva.;E S Platonov.
来源: Ontogenez. 2010年41卷2期107-13页
The action of two types of substances has been studied: 5-azadeoxycytidine and retinoic acid, which have a demethylation effect on DNA in the development process of diploid parthenogenetic mouse embryos. The effect of 5-azadeoxycytidine on hybrid mice (CBAxC57BL/6)F1 in vitro for 6 h, in the presence of single cell parthenogenetic embryos during the S-phase of the cell cycle has been studied. After developing to the blastocyst stage in vitro, parthenogenetic embryos were transplanted into the uterus of false pregnant females. It has been determined that a concentration of 0.1 microM 5-azadeoxycytidine activates embryonic development in the preimplantation period until the blastocyst stage (69% in experiment; 61% in the control) and during postimplantation, it increases the number of available space in the uterus for implantation (76% in experiment; 63% in the control). The effect of retinoic acid on parthenogenetic embryos from inbred C57BL/6 or CBA mice lines was studied by adding it to single cell embryos in a medium in vitro for 96 h. Treating parthenogenetic embryos C57BL/6 with retinoic acid concentrations 0.1 microM or 0.5 microM significantly increased the number of spaces for embryo implantation, 76% and 78% respectively, as against 57% for untreated embryos. Addition of similar doses of retinoic acid to the nutrient medium containing CBA parthenogenetic mouse embryos does not improve implantation (as with embryos C57BL/6), and a concentration of 2.0 microM is toxic to the embryos. During the period of postimplantation, parthenogenetic embryos of mouse lines C57BL/6 treated with retinoic acid just as the controls, did not develop to the somite stage. Mouse lines CBA had 45% of their embryos which were used as controls, developing to the advanced somite stages. However, the number of embryos treated with retinoic acid does not increase. Thus the treatment of two parthenogenetic embryos from inbred mice lines and their hybrids with compounds which demethylate DNA (5-azadeoxycytidine and retinoic acid) creates an opportunity for partial modulation of genomic imprinting and an increase in the survival rate of such embryos.

422. [Erythropoiesis-stimulating properties of an antiserotonin drug under cytostatic treatment conditions].

作者: E G Skurikhin.;O V Pershina.;N N Ermakova.;T V Andreeva.;E S Khmelevskaia.;O S Borsuk.;A M Dygaĭ.
来源: Eksp Klin Farmakol. 2010年73卷3期21-4页
Effect of the antiserotonin drug cyproheptadine on the erythropoiesis has been studied under conditions of the administration of cytostatics (fluoropyrimidine antimetabolite 5-fluorouracil (5-FU), alkylating agent cyclophosphane) with different mechanisms of action. It is established that the antiserotonin drug significantly accelerates regeneration of the erythroid hemopoietic branch, especially in the case of 5-FU. The depression of erythron under the conditions of cyclophosphane injections was retained. The erythropoiesis-stimulating effect of cyproheptadine is based on the restoration of the structural and functional organization of the bone marrow (formation of erythroid hemopoietic islets).

423. [A rare complication of imatinib mesylate therapy: drug-induced pneumonitis].

作者: O V Stakhina.;A G Turkina.;I E Kostina.;Iu B Kochkareva.
来源: Ter Arkh. 2010年82卷2期59-61页
The use of imatinib mesylate (Glivec) (Novartis Pharma AG, Switzerland) that is the drug of choice in treating patients with chronic myeloid leukemia (CML) has increased 7-year survival and improved the prognosis of the disease. The drug is generally tolerated well; the proportion of patients in whom imatinib treatment results in the development of toxic complications is small. Drug-induced interstitial pneumonitis associated with imatinib therapy is a rare complication that requires timely differential diagnosis, discontinuation of an inductor (imatinib), and altered further treatment policy.

424. [Vitamin K3-induced activation of molecular oxygen in glioma cells].

作者: N G Krylova.;T A Kulagova.;G N Semenkova.;S N Cherenkevich.
来源: Ukr Biokhim Zh (1999). 2009年81卷6期85-93页
It has been shown by the method of fluorescent analysis that the rate of hydrogen peroxide generation in human U251 glioma cells under the effect of lipophilic (menadione) or hydrophilic (vikasol) analogues of vitamin K3 was different. Analyzing experimental data we can conclude that menadione underwent one- and two-electron reduction by intracellular reductases in glioma cells. Reduced forms of menadione interact with molecular oxygen leading to reactive oxygen species (ROS) generation. The theoretical model of ROS generation including two competitive processes of one- and two-electron reduction of menadione has been proposed. Rate constants of ROS generation mediated by one-electron reduction process have been estimated.

425. [Cross-linked nucleic acids: formation, structure, and biological function].

作者: V A Efimov.;S V Fediunin.;O G Chakhmakhcheva.
来源: Bioorg Khim. 2010年36卷1期56-80页
Published data on the main types of reagents capable of introducing covalent interstrand cross links into nucleic acids (NA) are summarized in the present review. The reactivity of cross-linking agents, their preferred binding sites, and methods of determining the cross-link localization in a duplex are discussed. Cell response to DNA cross linking, namely, the blocking of replication and transcription, the initiation of reparation processes, and apoptotic death of the cell, are analyzed, as well as the use of cross-linking reagents in therapy and molecular biology.

426. [Antitumor properties of a liposomal form of a differentiation factor HLDF fragment in cultured cells].

作者: A V Bateneva.;O A Babenko.;E D Danilenko.;V D Poryvaev.;N N Karpyshev.;E P Goncharova.;I A Kostanian.;V I Masycheva.
来源: Eksp Klin Farmakol. 2010年73卷2期18-21页
Cytotoxic properties of a liposomal form of the HLDF6 hexapeptide, representing an HL-60 cell differentiation factor fragment, have been studied on a murine primary lymphosarcoma cell culture. It is established that the liposomal HLDF6 peptide is capable of inhibiting proliferation and enhancing death of the cells of both LS and RLS lymphosarcoma strains distinguished by their sensitivity to cytostatic agents. The effect of the preparation is determined by its antiproliferative and apoptogenic actions on the cells. Free HLDF6 peptide showed a lower cytotoxic activity with respect to the tumor cells as compared to the liposomal preparation.

427. [Evaluation of fludarabine-containing regimens versus immunochemotherapy for chronic lymphocytic leukemia].

作者: T P Zagoskina.;S B Tkachenko.;M E Golubeva.;A V Kudriavtseva.;N V Isaeva.;O V Malykh.
来源: Ter Arkh. 2010年82卷1期35-9页
to analyze the efficacy of RFC (rituximab, fludarabine, and cyclophosphan), FCM (fludarabine, cyclophosphan, and mitoxantrone), and FC (fludarabine and cyclophosphan) treatment programs in patients with chronic lymphocytic leukemia (CLL) in an open-labeled comparative controlled investigation.

428. [Effectiveness of gefitinib (Iressa) as first-line therapy for inoperable non-small-cell lung cancer with mutated EGFR gene (phase II study)].

作者: V M Moiseenko.;S A Protsenko.;I I Semenov.;F V Moiseenko.;E V Levchenko.;A S Barchuk.;D E Matsko.;A O Ivantsov.;A G Ievleva.;N V Mitiushkina.;A V Togo.;E N Imianitov.
来源: Vopr Onkol. 2010年56卷1期20-3页
Tumor regression was reported in 20-30% of patients with inoperable non-small-cell lung cancer (NSLC) following standard first-line chemotherapy. Clinical trials with second-line gefitinib (Iressa) showed a strikingly high response in patients with mutated EGFR. However, clinical experience with gefitinib as first-line therapy had been limited to small-scale trials mostly among subjects of Asian origin. Our study was not associated with the drug manufacturer and included 25 chemotherapy-naive patients with mutated EGFR inoperable lung adenocarcinoma. Standard dose was 250 mg/day. Complete response was observed in 1 patient (4%), partial--11 (44%), sustained stabilization--13 (52%); median time until tumor progression--186 days. Median overall survival failed to be registered within the duration of the study. Among most frequent side-effects were skin rash (19; 76%) and diarrhea (14; 56%): marked side-effect -toxicity grade III (4; 16%). Gefitinib appeared highly efficient and tolerable and may be recommended as first-line treatment of mutated EGFR inoperable NSLC.

429. [Multidrug resistance increase of tumor cells at the effect of radiation and phorbol ether depends on protein kinase C and reactive oxygen species].

作者: L Kh Eĭdus.;M O Emel'ianov.;A F Korystova.;L N Kublik.;V V Shaposhnikova.;Iu N Korystov.
来源: Radiats Biol Radioecol. 2010年50卷1期37-41页
The effects of phorbol ether (PMA) and ionizing radiation on multidrug resistance (MDR) of human larynx cancer cells HEp-2 and the dependences of these effects on protein kinase C (PKC) activity and reactive oxygen species (ROS) production were studied. MDR was determined by transport rate of rhodamine 123 off cells and production of ROS in cells was measured by means of 2'7'-dichlorodigidrofuorescein oxidation to fluorescent 2',7'-dichlorofluorescein. ROS production was increased in cells at PMA treatment. This increase was caused by PKC dependent activation of NADPH-oxidase because the ROS increase suppressed completely with PKC and oxidase inhibitors. It was shown that tumor cell MDR was increased 16 h after PMA (100 nM) and radiation (1 Gy) treatment. The MDR increase depends on PKC activity and ROS increase in cells at both influences since PKC inhibitor and antioxidant, lipoic acid suppressed MDR increase. The obtained data are in accordance with hypothesis about the necessity of activation both signalization and stress reaction for initiation of transcription of transport protein genes which responsible for MDR of tumor cells.

430. [New conception of surgery in Peyronie's disease].

作者: V A Kovalev.;S G Karaguzhin.;S V Koroleva.;A N Abdulkhamidov.;M G Levadnaia.
来源: Urologiia. 2009年5期45-8页
Clinical experience of the authors on patients with Peyronie's disease shows that intraplaque therapy with verapamil and interferon and a malignant course of the disease can result in shortening of the penis (4 and 1 cases were observed, respectively). The length of the penis diminished by 30 to 50%. The patients were treated with graft corporoplasty with endoprosthesis of the penis. Basing on the experience with these cases the following conclusions were made: intraplaque injections are hard to perform and can provoke progression of the disease; long-term conservative treatment for stabilization of the active stage of Peyronie's disease can lead to shortening of the penis; in malignant course of the disease and progressive shortening of the penis surgical policy is justified despite "acute" phase of the disease.

431. [Effects of indol-3-carbinol and epigallocatexin-3-gallate on alteration and reparation in affected urethra of experimental animals].

作者: V N Pavlov.;A A Kazikhinurov.;R I Safiullin.;R A Kazikhinurov.;K G Kutushev.;I R Valiev.
来源: Urologiia. 2009年5期29-33页
The effects of indol-3-carbinol and epigallocatexin-3-gallate on alteration and reparation in the urethra were studied in 30 male Shinshilla rabbits. From the observation day 2 the rabbits were fed with indol-3-carbinol and epigallocatexin-3-gallate in addition to standard diet. Microcirculation was assessed with a laser analyzer of capillary circulation LAKK-01 which detected earlier recovery of microcirculation in the group of the animals fed with indol-3-carbinol and epigallocatexin-3-gallate. Thus, antioxidant and antiproliferative properties of indol-3-carbinol and epigallocatexin-3-gallate improve alteration and reparation in affected urethra due to development of soft elastic connective tissue with better capillary circulation.

432. [Antiangiogenic therapy for choroidal neovascularization induced by age-related macular degeneration (preliminary communication)].

作者: S E Avetisov.;V R Mamikoian.;B K Surguch.;S V Sdobnikova.
来源: Vestn Oftalmol. 2009年125卷6期3-8页
Specific antiangiogenic therapy for exudative age-related macular degeneration (AMD) aims at suppressing the growth of choroidal neovascularization. Bevacizumab, an antibody to vascular endothelial growth factor, is one of the agents that specifically inhibit angiogenesis. The results of using bevacizumab in choroidal neovascularization induced by AMD are analyzed. Bevacizumab was administered into the vitreous body of 56 patients (60 eyes) in a dose of 1.25 mg; its injections were repeated if the need arose in disease recurrence. The follow-up time was 6 to 12 months. Following 6 months, visual preservation not below the baseline level was noted in 86% of cases; in this period a significant visual improvement was stated in 46% of cases. Fluorescence angiography showed decreased dye leakage in most cases, which correlated with the lower retinal thickness revealed by optical coherence tomography. There were no serious complications. Intravitreal administration of bevacizumab is the method of choice in treating exudative AMD and requires further investigation.

433. [Value of dynamic study of cytokines in serum and leukocytes in patients with acute leukemia].

作者: A B Makeshova.;A A Levina.;Iu I Mamukova.;N S Melik-Nubarov.;M M Tsibul'skaia.;V G Savchenko.
来源: Klin Lab Diagn. 2009年12期19-24页
A number of interleukins and iron metabolic parameters were studied in acute leukemia over time. Regulation of hemopoiesis and the cytokine network has been found to be impaired, which appears as the increased synthesis of ferritin and hepsidin by macrophages that are activated by imbalance in the cytokine network. A severe impairment of the parameters responsible for a regulatory process is shown to occur in leukemia. There is no complete normalization of these parameters during hematological remission, which is likely to lead to a further relapse of leukemic process.

434. [Combination herbal preparation in the prevention and treatment of cytotoxic therapy-induced mucositis].

作者: T N Popova.;T P Spirina.;E A Kuzevanova.
来源: Vestn Otorinolaringol. 2009年6期80-2页

435. [Hairpin oligonucleotides as actinomycin carriers].

作者: N L Vekshin.;I V Savintsev.
来源: Biofizika. 2009年54卷6期1037-41页
The redistribution of actinomycin from hairpin oligonucleotide HP1 to dissolved DNA and DNA inside the cell has been discovered and investigated. It was found that the penetration of the antibiotic in a complex with HP1 into cancer cells takes place more effectively than that of the antibiotic separately. It is suggested that hairpin oligonucleotides can serve as molecular carriers of heterocyclic antibiotics to cancer cells.

436. [Retinoic acid as a regulator of planarian morphogenesis].

作者: O N Ermakova.;A M Ermakov.;Kh P Tiras.;V V Lednev.
来源: Ontogenez. 2009年40卷6期449-55页
The effect of retinoic acid on regeneration of two species of asexual planarian races, Girardia tigrina and Schmidtea mediterranea, was studied. It was established that retinoic acids at physiological concentrations (10(-7)-10(-10) M) inhibit the regeneration of the head part of planarians but have no effect on tail blastema growth. It is shown that regeneration of the head part is inhibited as a result of arrest of the cell cycle of neoblasts, proliferating stem cells, during the transition from the G1/G0 to the S phase. Thus, the morphogenetic role of retinoic acids in planarians, primitive bilaterally symmetrical animals, has been demonstrated.

437. [Acute and postponed action of adriamycin on the contractile function and antioxidant status of the myocardium].

作者: V I Kapel'ko.;V L Lakomkin.;G G Konovalova.;V G Tsyplenkova.;A K Tikhaze.;V Z Lankin.
来源: Kardiologiia. 2010年50卷12期45-51页
Functional, biochemical and morphological studies of rat cardiac muscle after single injection of adriamycin (2.2 mg/kg) were carried out. The myocardium was taken for studies in 2 hours and in 2-3 weeks after adriamycin injection. The isolated heart was perfused retrogradely with Krebs solution and left ventricular isovolumic pressure and perfusion pressure were continuously monitored. Two-fold increase in perfusion rate was accompanied by raised developed pressure, heart rate and perfusion pressure which in the given conditions reflected a tone of coronary vessels. The cardiac contractile function of rats that received adriamycin 2 hours before, remained unaltered as compared to control group, however, perfusion pressure was raised by 26%. These hearts responded to H2O2 introduction (100 microM) into coronary vessels by more profound fall in developed pressure, which fell to 31 +/- 8% after 40 minutes vs. 61 +/- 5% in the control group (p<0.01). In two-three weeks after adriamycin injection, both cardiac contractile function and its responsiveness to oxidative stress induced by H2O2 introduction did not differ from the control, however, perfusion pressure remained elevated and this was accompanied by slowed myocardial relaxation. The myocardial concentration of malonic dialdehyde was moderately increased in adriamycin-treated group in both terms while the activity of antioxidant enzymes (SOD, GPHx and catalase) remained unaltered. Results showed an absence of the direct connection between myocardial antioxidant status and the contractile function changes at adriamycin action.

438. [Genetic heterogeneity of heat shock protein synthesis as a factor determining the resistance of mammalia to stressors].

来源: Tsitologiia. 2010年52卷12期1016-23页
The relationship between the levels of 70 kDa family heat shock protein (Hsp) synthesis and lymphocyte sensitivity to stressors was investigated. Lymphocyte cultivation in mitogen deprived culture medium and (or) the cell treatment with alkylating agents have been used as a stress challenge. On the model of two inbred murine strains genetically contrasting by the sensitivity to alkylating agents we succeeded in demonstration that the basic level of Hsp synthesis depends on genotype. The quantity Hsp mRNA, as well as the intracellular level of the proteins were significantly higher in BALB/c than in C57BL/6 mice. The mice characterized by higher Hsp levels demonstrated higher resistance to alkylating agent action. The induction of surplus amount of Hsp by heat shock increased the cell resistance to the alkylating agent melphalan. Lymphocyte isolated from high Hsp producers, BALB/c mice, were more resistant to apoptotic signals induced by mitogen deprivation.

439. [Preclinical trials of L-lysine-alpha-oxidase, an antitumor enzyme].

作者: V M Podboronov.;A E Kuzovnikov.;A K Zaĭtseva.;I P Smirnova.
来源: Antibiot Khimioter. 2010年55卷9-10期33-6页
The effect of L-lysine-alpha-oxidase from a representative of the Trichoderma genus on the humoral immune response to protein antigens was studied. It was shown that repeated fife-fold intravenous administrations of L-lysine-alpha-oxidase in a dose of 35 U/kg had no depressive action on the humoral immunity. The enzyme had no suppressive effect on the delayed type hypersensitivity to xenogenous erythrocytes. The use of L-lysine-alpha-oxidase in a therapeutic dose or in a twice as higher dose had no reliable effect on the leukocyte migration capacity vs. the control.

440. [Experimental study on cytoflavin pharmacological correction of cyclophosphamide-induced toxic affections of the liver and kidneys].

作者: V A Kashuro.;S I Glushkov.;L V Minaeva.;T M Novikova.
来源: Antibiot Khimioter. 2010年55卷9-10期29-32页
The experimental data concerning the influence of repeated administration of cyclophosphamide in a total dose of 200 mg/kg on the glutathione metabolism and lipid peroxidation in the liver and kidneys of albino noninbred rats are presented. The possibility of the natural cytoprotection system correction by the use of cycloflavin was shown. The cytoprotection action mechanisms of the pharmacological agent are discussed.
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