当前位置: 首页 >> 检索结果
共有 2616 条符合本次的查询结果, 用时 4.9756454 秒

241. IMMUNOMODULATORY EFFECT OF ALLOGENEIC MESENCHYMAL STEM CELLS OF RATS.

作者: I E Trubitsyna.;N A Onishchenko.;A V Lyundup.;O V Knyazev.;A S Gulyaev.;O S Vasnev.;Z M Abdulatipova.;A V Smirnova.;Yu M Orlova.;G A Drozdova.
来源: Eksp Klin Gastroenterol. 2016年11期59-63页
Recently, more and more attracted the attention of cell therapy, which requires a study of the efficacy and safety of allogeneic MSCs transplantation in acute and chronic inflammatory reactions. The aim of our study was to examine the effectiveness of transplantation of allogeneic mesenchymal bone marrow stromal cells for the healing of surgical wounds the glandular stomach in rats.

242. FUNCTIONAL STATE OF MULTIPOTENT MESENCHYMAL STROMAL CELLS DURING MODELING THE EFFECTS OF MICROGRAVITY.

作者: A Yu Ratushnyy.;L B Buravkova.
来源: Aviakosm Ekolog Med. 2016年50卷5期24-29页
Multipotent mesenchymal stromal cells (MMSCs) are essential for regeneration and tissue homeostasis. The investigation was focused on the functional state of MMSCs isolated from human adipose tissue and exposed to simulated microgravity in vitro by RPM (random position machine). The results point to an increase of proliferative potential, decrease of the lisosomal compartment activity, and reductions in cell size and granularity. Evaluation of the paracrine activity in relatively static control revealed increased IL-8 production and decreased IL-6 production. *Our .data supplement previous findings and allow conclude about universality of the mesenchymal cell reaction to simulated microgravity. The investigation did not detect signs of cell stress in this environment.

243. STATUS OF THE SYSTEM OF SIGNALING PATTERN RECOGNITION RECEPTORS OF MONOCYTES AND GRANULOCYTES IN COSMONAUTS' PERIPHERAL BLOOD BEFORE AND AFTER LONG-DURATION MISSIONS TO THE INTERNATIONAL SPACE STATION.

作者: S A Ponomarev.;T A Berendeeva.;S A Kalinin.;A V Muranova.
来源: Aviakosm Ekolog Med. 2016年50卷5期18-23页
The system of signaling pattern recognition receptors was studied in 8 cosmonauts aged 35 to 56 years before and after (R+) long-duration missions to the International space station. Peripheral blood samples were analyzed for the content of monocytes and granulocytes that express the signaling pattern recognition Toll- like (TLR) receptors localized as on cell surface (TLR1, TLR2, TLR4, TLR5, TLR6), so inside cells (TLR3, TLR8, TLR9). In parallel, serum concentrations of TLR2 (HSP60) and TLR4 ligands (HSP70, HMGB1) were measured. The results of investigations showed growth of HSP60, HSP70 and HMGB1 concentrations on R+1. In the;majority of cosmonauts increases in endogenous ligands were followed by growth in the number of both monocytes and granulocytes that express TLR2 1 TLR4. This consistency gives ground to assume that changes in the system of signaling pattern recognition receptors can stem .from the predominantly endogenous ligands' response to the effects of long-duration space flight on human organism.

244. [The Cell Therapy in Traumatic Spinal Cord Injury].

作者: A D Voronova.;O V Stepanova.;A V Chadin.;I V Reshetov.;V P Chekhonin.
来源: Vestn Ross Akad Med Nauk. 2016年71卷6期420-6页
The opportunities and the most promising ways of using cellular technology in traumatic spinal cord injury are considered in this review. A large number of experimental and clinical studies with the use of different types of cells: embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, Schwann cells, olfactory mucosa cells, and others – was conducted. The use of these types of cells in traumatic spinal cord injury treatment often demonstrated a positive therapeutic effect: the motor and sensory function recovery of the spinal cord. However, some types of cell preparations involve some methodological and ethical problems; some types of cell therapies are ineffective or give rise to side effects. These factors complicate the selection of optimal cell therapy for the traumatic spinal cord injury treatment. The most promising cells seem to be the cells of the olfactory mucosa. Getting the olfactory mucosa is considered to be a feasible and safe procedure for patients. The clinical application of the cells of the olfactory mucosa is effective in motor function recovery due to remyelination and axonal regeneration after spinal cord injury. These cells are tissue-specific and autologous since they can be obtained from a patient with spinal cord injury, and after cultivation, expansion, and directed differentiation they can be transplanted to the same patient. The presented benefits of olfactory mucosa cells open up the possibility for its clinical application in the cell therapy.

245. [The Use of Stem Cells in the Treatment of Intervertebral Disc Degeneration].

作者: V A Byvaltsev.;I A Stepanov.;L A Bardonova.;E G Belykh.
来源: Vestn Ross Akad Med Nauk. 2016年71卷5期359-66页
The paper presents a review of current data on the use of stem cells in the treatment of intervertebral disc degeneration. Acute spinal pain is often a consequence of the pathology affecting the intervertebral disc. Many applied therapeutic techniques do not provide effective results as expected because most of them address symptoms, but do not treat the underlying disease. We have outlined current findings on the molecular mechanisms of intervertebral disc degeneration, analyzed international experimental studies demonstrating the feasibility of a stem cell therapy for intervertebral disc degeneration. The conducted studies reported on the clinical application of mesenchymal stem cells or stem cells derived from adipose, synovium, and bone marrow tissue. The most pressing and undetermined issues that require further experimental and clinical studies are indicated and defined in the article.

246. [Experimental study of the effect of combined transplantation of multipotent mesenchymal stromal cells and hematopoietic stem cells on the regeneration of the intestinal epithelium in the conditions of influence of extreme factors].

作者: I U Maklakova.;D U Grebnev.;A P Yastrebov.
来源: Adv Gerontol. 2016年29卷3期433-436页
The study examined the effect of combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells on the regeneration of the intestinal epithelium in the conditions of influence of extreme factors. Obtained in physiological conditions and in the conditions of influence of extreme factors combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells derived from mature and old animals is accompanied by increased content of epithelial cells of the crypts of the jejunum. In physiological conditions in mature animals, this effect is realized through the increase of proliferative activity of epithelial cells, while the old - through the inhibition of apoptosis. In the conditions of influence of extreme factors of the increase in the content of the cell population of the crypts in mature animals is achieved by increasing the proliferative activity and the inhibition of apoptosis of epithelial cells in the old organism through inhibition of apoptosis.

247. [Therapeutic potential of human mesenchymal stromal cells secreted components: a problem with standartization].

作者: G D Sagaradze.;O A Grigorieva.;A Yu Efimenko.;A A Chaplenko.;S N Suslina.;V Yu Sysoeva.;N I Kalinina.;Zh A Akopyan.;V A Tkachuk.
来源: Biomed Khim. 2015年61卷6期750-9页
Regenerative medicine approaches, such as replacement of damaged tissue by ex vivo manufactured constructions or stimulation of endogenous reparative and regenerative processes to treat different diseases, are actively developing. One of the major tools for regenerative medicine are stem and progenitor cells, including multipotent mesenchymal stem/stromal cells (MSC). Because the paracrine action of bioactive factors secreted by MSC is considered as a main mechanism underlying MSC regenerative effects, application of MSC extracellular secreted products could be a promising approach to stimulate tissue regeneration; it also has some advantages compared to the injection of the cells themselves. However, because of the complexity of composition and multiplicity of mechanisms of action distinguished the medicinal products based on bioactive factors secreted by human MSC from the most of pharmaceuticals, it is important to develop the approaches to their standardization and quality control. In the current study, based on the literature data and guidelines as well as on our own experimental results, we provided rationalization for nomenclature and methods of quality control for the complex of extracellular products secreted by human adipose-derived MSC on key indicators, such as "Identification", "Specific activity" and "Biological safety". Developed approaches were tested on the samples of conditioned media contained products secreted by MSC isolated from subcutaneous adipose tissue of 30 donors. This strategy for the standardization of innovative medicinal products and biomaterials based on the bioactive extracellular factors secreted by human MSC could be applicable for a wide range of bioactive complex products, produced using the different types of stem and progenitor cells.

248. [Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration].

作者: S Yu Ivanov.;A P Bonartsev.;Yu V Gazhva.;I I Zharkova.;R F Mukhametshin.;T K Mahina.;V L Myshkina.;G A Bonartseva.;V V Voinova.;N V Andreeva.;E A Akulina.;E S Kharitonova.;K V Shaitan.;A A Muraev.
来源: Biomed Khim. 2015年61卷6期717-23页
Bone tissue damages are one of the dominant causes of temporary disability and developmental disability. Currently, there are some methods of guided bone regeneration employing different osteoplastic materials and insulation membranes used in surgery. In this study, we have developed a method of preparation of porous membranes from the biopolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), produced by a strain of Azotobacter chroococcum 7B. The biocompatibility of the porous membranes was investigated in vitro using mesenchymal stem cells (MSCs) and in vivo on laboratory animals. The cytotoxicity test showed the possibility of cell attachment on membrane and histological studies confirmed good insulating properties the material. The data obtained demonstrate the high biocompatibility and the potential application of insulating membranes based on PHBV in bone tissue engineering.

249. [Molecular mechanisms of niclosamide antitumor activity].

作者: E Yu Moskaleva.;V G Perevozchikova.;A S Zhirnik.;S E Severin.
来源: Biomed Khim. 2015年61卷6期680-93页
In this review the recent data regarding the antitumor activity of niclosamide and the molecular mechanisms of its antitumor activity are presented. Niclosamide has been used in the clinic for the treatment of intestinal parasite infections. In recent years in several screening investigations of various drugs and chemical compounds niclosamide was identified as a potential anticancer agent. Niclosamide not only inhibits the Wnt/β-catenin, mTORC1, STAT3, NF-κB and Notch signaling pathways, but also targets mitochondria in cancer cells to induce growth inhibition and apoptosis. A number of studies have established the anticancer activity of niclosamide in both in vitro and in vivo in xenotransplantation models using human tumors and immunodeficient mice. It is important that niclosamide is active not only against tumor cells but also cancer stem cells. Normal cells are resistant to niclosamide. The accumulated experimental data suggest niclosamide is a promising drug for the treatment of various types of cancer.

250. [Mesenchymal stem cells expressing cytosine deaminase inhibit growth of murine melanoma B16F10 in vivo].

作者: L S Krasikova.;S S Karshieva.;I B Cheglakov.;A V Belyavsky.
来源: Mol Biol (Mosk). 2015年49卷6期1007-15页
The aim of this study was to estimate the efficacy of mesenchymal stem cell-based suicide gene therapy in mice bearing murine melanoma B16F10. Adipose mesenchymal stem cells (MSCs) were transfected with plasmid constructs expressing cytosine deaminase fused with uracil phosphoribosyltransferase (CDA/UPRT) or CDA/UPRT fused with HSV-1 tegument protein VP22 (CDA/UPRT/VP22). In this study, we demonstrate that direct intratumoral transplantation of MSCs expressing CDA/UPRT or CDA/UPRT/VP22 followed by systemic administration of 5-fluorocytosine (5-FC) results in a significant inhibition of tumor growth. There was a 53% reduction in tumor volume in mice treated with CDA/UPRT-MSCs and 58% reduction in tumor volume in mice treated with CDA/UPRT/VP22-MSCs as compared with control animals transplanted with B16F10 melanoma alone. Injection of CDA/UPRT-MSC and CDA/UPRT/VP22-MSC prolonged the life span of mice bearing B16F10 melanoma by 15 and 26%, respectively. The data indicate that in murine B16F10 melanoma model, MSCs encoding CDA/UPRT suicide gene have a significant antitumor effect.

251. [Homogeneous and heterogeneous 3D melanoma models in vitro].

作者: O F Kandarakov.;M V Kalashnikova.;A A Vartanian.;A V Belyavsky.
来源: Mol Biol (Mosk). 2015年49卷6期998-1001页
Growth of malignant tumors occurs in three-dimensional space and depends on a presence of stromal component, which performs critical functions of tumor cell protection and growth support. Therefore, development and analysis of tumor models in 3D cell cultures in vitro, including co-culture systems, presents a significant interest. In this study, the results of 3D culturing of two human melanoma cell lines using the hanging drop method, with or without human mesenchymal stem cells (MSCs), are presented. Melanoma lines were shown to behave differently in 3D cultures; in particular, Mel Cher melanoma cells have the ability to form uniform spheroids within 24 h, whereas MeWo cells under similar conditions failed to form spheroids even after 2 days of culture. However, co-culturing of melanoma cells with MSCs resulted in formation of compact 3D cell spheroids in both cases. Visualization of MeWo cells and MSCs in the mixed spheroids using fluorescent dyes revealed certain clustering of melanoma cells. The observed properties of melanoma cells in homogeneous and heterogeneous spheroids may be used in the complex analysis of results of testing of antimelanoma chemotherapy drugs and evaluation of their therapeutic properties.

252. [Poised RNA polymerase II and master regulation in Metazoa].

作者: K N Kashkin.;E D Sverdlov.
来源: Mol Biol (Mosk). 2015年49卷6期905-14页
This review is devoted to the mechanisms of transcriptional pause and poised state of RNA polymerase II. Features of poised promoters and chromatin are considered in brief. Role of regulated transcriptional pause as discrete and important stage in regulation of master genes that determine stem-cell differentiation, cell lineage and development in Metazoa is discussed. This work was supported by the Russian Science Foundation (project No. 14-50-00131).

253. [Orbital Adipose Tissue: Just a Fat Pad or Terra Incognita in Ophthalmology].

作者: S A Borzenok.;D S Afanasyeva.;M B Gushchina.
来源: Vestn Ross Akad Med Nauk. 2015年4期464-7页
Our understanding of the role of adipose tissue has been completely changed during the past decades. The knowledge of its contribution to endocrine and immune pathways opened the new insights on the pathogenesis and therapy of many diseases and new perspectives for the regenerative medicine. The further researches should be provided to study anatomy and functions of local fat depots in more details. Of the most interest is the orbital adipose tissue due to its origin from the neural crest. This review represents the current data about anatomy, structure, cell composition and biochemistry of orbital fat. The main attention is put to such cell types as adipocytes and adipose derived mesenchymal stem cells. The foreign authors' findings on such characteristics of stem cells from orbital adipose tissue as CD markers and differential capacity are reviewed. The found evidences of interaction between orbital adipose tissue, eyeball and associated structures allow us to hypothesize that this fat depot may contribute to various ocular pathology. In this paper, we outlined the possible directions for further investigation and clinical application of orbital fat and cells its composing in ophthalmology, reconstructive and plastic surgery and regenerative medicine.

254. [Effect of extracellular matrix components on adhesion of bone marrow multipotent mesenchymal stromal cells to polytetrafluoroethylene].

作者: A A Karpenko.;I A Rozanova.;O V Poveshchenko.;A P Lykov.;N A Bondarenko.;I I Kim.;Iu V Nikonorova.;N A Podkhvatilina.;D S Sergeevichev.;I V Popova.;V I Konenkov.
来源: Angiol Sosud Khir. 2015年21卷4期178-84页
Search for new bioengineering materials for creation of small-diameter vascular grafts is currently a priority task. One of the promising trends of creating tissue engineering constructions is coating the internal layer of implants made of polytetrafluoroethylene (PTFE) with autologous mesenchymal multipotent stromal cells. In the study we assessed the ability of separate components of the extracellular matrix such as fibronectin, type I collagen and type IV collagen to influence adhesion, proliferation and morphology of mesenchymal multipotent stromal cells being cultured on PTFE. Bone marrow multipotent stromal cells taken from second-passage Wistar rats in the amount of 106 per 1 cm2 were applied onto PTFE. We used the following variants of preliminary treatment of the material prior to seeding: fibronectin with type I collagen, fibronectin with type IV collagen, fibronectin with a mixture of type I and IV collagens, as well as a control group without coating. After six weeks of cell growth on PTFE patches the samples were subjected to fixation in 10% formalin followed by haematoxylin-eosin stain and morphometric assessment of adhered cells by calculation using the software AxioVision (Carl Zeiss), assessing the number of cells, area of the cellular monolayer, dimensions and ratios of the area of separate cells and the area of cellular nuclei. The maximal area of the monolayer from mesenchymal multipotent stromal cells on the PTFE surface was revealed while culturing with a mixture of fibronectin and type I and IV collagens. Cell colonization density while treatment of the synthetic material with mixtures of fibronectin with type I collagen, type IV collagen and type I and IV collagens demonstrated the results exceeding the parameters of the control specimen 5-, 2.5- and 7-fold, respectively. Hence, extracellular matrix components considerably increase enhance adhesion of cells to PTFE, as well as improve formation of a monolayer from mesenchymal multipotent stromal cells.

255. [MODIFICATION OF MESENCHYMAL STEM CELLS AS A WAY TO IMPROVE THE EFFECTIVENESS OF CELL THERAPY OF ISCHEMIC MYOCARDIAL INJURY].

作者: A A Karpov.;A V Drahova.;D V Buslova.;D Yu Ivkin.;O M Moiseeva.;M M Galagudza.
来源: Ross Fiziol Zh Im I M Sechenova. 2015年101卷9期985-98页
Acute myocardial infarction remains to be one of the most important problems of health care. Cardiac cell therapy is a new therapeutic strategy focused on repair of the injured cardiac muscle. Mesenchymal stem cells (MSC) are considered as the most suitable candidates for cardiac cell therapy. MSC transplantation in the myocardium after ischemic injury has been shown to be cardioprotective in animal models and clinical trials. However, the beneficial effects of MSC in humans are limited because of both poor survival and impaired function of the cells in ischemic tissue. To address these issues, a number of approaches to the modification of MSCs with the aim to improve their survival and proliferation, reduce the immune reaction, enhance transdifferentiation, and optimize the profile of secreted paracrine factors have been tested. In this review, we provide detailed discussion of different methods of MSCs modification, including targeted gene overexpression, conditioning of MSCs using physical and chemical factors, and application of multicellular units for transplantation. The effectiveness of these strategies in preclinical studies is also discussed.

256. [ADULT STEM CELLS AND CELLS OF MALIGNANT ORIGIN. PART I].

作者: E D Sverdlov.;V V Pleshkan.;I V Alekseenko.;T V Vinogradova.;E P Kopantzev.;D A Didich.
来源: Mol Gen Mikrobiol Virusol. 2015年33卷3期3-8页
Recent data on adult stem cells are reviewed. According to the present dominant paradigm, it is most probable that cancer predisposition arises or cancer is initiated in these cells.

257. [Prospects for application of stem cells from adipose tissue in treating erectile dysfunction].

作者: M E Chaliy.;P V Glybochko.;M V Epifanova.;A O Krasnov.
来源: Urologiia. 2015年6期98-103页
Although phosphodiesterase type 5 inhibitors are the first-line treatment of ED, some patients remain unresponsive to any pharmacotherapy. To date, the use of stem cells is considered as a new treatment modality for erectile dysfunction. The validity of this assumption has been experimentally proved in preclinical and clinical studies. The main focus is on the stem cells derived from adipose tissue, due to advantages over other sources.

258. [ANGIOGENESIS IN THE TISSUES AFTER THE INJECTION OF STROMAL STEM CELLS OF BONE MARROW ORIGIN CLOSE TO THROMBOSED VEIN IN AN EXPERIMENT].

作者: I V Maiborodin.;V V Morozov.;Ya V Novikova.;V A Matveyeva.;L V Artemiyeva.;A L Matveyev.;R V Maslov.;N V Onopriyenko.;G A Chastikin.
来源: Morfologiia. 2015年148卷4期12-8页
The effects of the injection of autologous multipotent stromal stem cells of bone marrow origin (MSSCBM) (mesenchymal stem cells) with green fluorescent protein gene, additionally marked with DAPI nuclear stain, close to a thrombosed hindlimb vein, were studied by fluorescent microscopy in adult male Wag rats (n = 214). The control groups consisted of intact rats (n = 12), animals with venous thrombosis without the injection of MSSCBM (n = 71) and rats that received paravasal injection of MSSCBM, but without preliminary modeling of venous thrombosis (n = 72). It was found that MSSCBM participated in the development of granulation tissue at the site of surgical intervention performed during the modeling of thrombosis. The rapid development of granulation tissue at the site of surgical trauma may contribute to faster wound clearance from detritus, nonviable tissue and antigenic substances, early onset of tissue repair processes and rapid healing. Restoration of blood flow in the tissue region of a thrombosed vein began later than after the intravenous injection of MSSCBM.

259. [CHANGES IN THE NUMBER OF REGENERATING MYELINATED FIBERS IN INJURED NERVE OF THE RAT AFTER ALLOTRANSPLANTATION OF THE DISSOCIATED CELLS OF THE EMBRYONIC CNS ANLAGES].

作者: Ye S Petrova.;Ye N Isayeva.
来源: Morfologiia. 2015年148卷4期7-11页
The study was conducted on 6 female and 36 male adult Wistar rats to compare the effects of dissociated cells derived from different embryonic CNS anlages, on the growth of regenerating nerve fibers in the damaged nerve of the recipient. After the sciatic nerve was damaged by ligation, part of the animals received the injection into the proximal portion of the nerve with a suspension of the cells obtained by dissociation of the fragments of spinal cord or forebrain vesicle taken from rat embryos at Day 15 of development. The analysis of transverse semithin sections of the distal part of the nerves was performed 21 and 60 days after surgery. It was found that the number of regenerating myelinated nerve fibers was increased 60 days after the injection of dissociated embryonic spinal cord cells, but not the neocortical cells into the damaged nerve of the recipient.

260. [Own Chemiluminescence of Planarian Neoblasts during Regeneration].

作者: H P Tiras.;S V Gudkov.;V I Emelyanenko.;K B Aslanidi.
来源: Biofizika. 2015年60卷5期975-80页
We investigated the kinetics of the luminescence induced by reactive oxygen species in planarians during regeneration process. It was found that regeneration is accompanied with changes in the concentration of reactive oxygen species correlating with energy-intensive processes such as oxidative stress, caused by damage to cell membranes in the dissection of the planarian, phagocytosis of dying cells and mitosis of neoblasts. We showed for the first time that there is an opportunity of registering the physiological state of pluripotent stem cells at the level of the organism in vivo.
共有 2616 条符合本次的查询结果, 用时 4.9756454 秒