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2481. [The activity and isoenzyme spectrum of esterases in erythroid and myeloid cells of rabbit bone marrow].
In erythroid and myeloid cells of rabbit bone marrow the total activity of esterases and their isozyme spectrum in polyacrylamide gel disc electrophoresis were studied. Higher esterase activity was observed in the myeloid cells as compared with erythroid ones. In the isozyme spectrum of esterases from the myeloid cells an additional cathode fraction was observed, which was not found in extracts of erythroid cells. Experiments with inhibitors showed that the enzymes studied were carboxyl- and acetylesterases.
2482. [Influence of 5-methoxytryptamine, cystamine, and aminopropyl-aminoethylthiophosphate on the radiosensitivity of hematopoietic stem cells].2483. [Foci of lymphocyte-like cells in the bone marrow of irradiated mice].2484. [Reimmigration of bone marrow stem cells in mice in radiation experiments].2485. [Ability of antilymphocyte sera to counteract the allogeneic inhibition of hematopoietic stem cells].2486. [Cellular composition of the bone marrow in cows].2487. [Functional dimorphism in satellite chromosomes in hamadryas baboons].
Dimorphism of satellite chromosomes observed in bone marrow of female as well as male Papio hamadryas, and in continuous cultures of the monkey haemopoetic cells is described. Most frequently the observed dimorphism is expressed in different lengths of satellite arms. The structural dimorphism of satellite chromosomes seen in Macaca, Papio and other genera may seem to originate from different degrees of functional activity of these chromosomes. The active homologue is longer since its satellite arm is less spiralized.
2488. [Effect of the lining of the thymus gland on hematopoietic stem cell kinetics in a suspension of embryonic mouse liver].
A study was made of the kinetics of hemopoietic stem cells in the organic culture of a suspension of mouse embryonic liver cultivated directly on the filters and on the preliminarily grown embryonic thymic feeder. It was shown that stem hemopoietic cells were retained in the course of one month of cultivation in the embryonic liver suspensions; during the first two weeks hemopoiesis in the suspension practically failed to differ from such in the fragments of the same age and cultivation period, whereas during the subsequent periods hemopoiesis proved to be more active in the suspension cultivated on thymic feeder. A possible participation of the thymus in the hemopoiesis is discussed.
2489. [Hematopoiesis recovery in irradiated mice during treatment with RES-seeking dyes].2490. [Radioresistance recovery and hematopoietic stem cells].2491. [Inactivation of hematopoietic stem cells by means of lymphocytes depending upon the genotype of the interacting cells].
Inoculation of a mixture of bone marrow cells with allogeneic lymphocytes into irradiated mice of inbred strains or into F1 hybrids results in the depression of bone marrow cell proliferation in the spleen of the recipient: the effect of inactivation of nonsyngeneic stem cells. The inactivation of stem cells by allogeneic lymphocytes can be detected in all tested combinations of mice strains - donors of lymphocytes and bone marrow cells and mice - recipients but the degree of inactivation differs and depends on the genotype of cell donors rather than on the genotype of the recipient. Lymphocytes of some mice strains (haplotypes H-2k and H-2a) are more active killers of bone marrow cells as compares with lymphocytes of other strains (hyplotypes H-2b and H-2d). Probably, the degree of stem cells inactivation by lymphocytes depends on the differences of their histocompatibility in H-2 system.
2492. [Current possibilities for the clinical transplantation of hematopoietic tissue].2493. [Cloning stem cells in the bone marrow of irradiated mice].
Different amount of intact or irradiated bone marrow from syngenous donors was administered to mice irradiated with a lethal dose. There was revealed a linear dependence of the number of the 8-9-day colonies grown in the bone marrow of the femur on the amount of the administered cells, and an exponential dependence on the irradiation dose. Regularity of the stem cell cloning in the bone marrow was analogous to such in the spleen. Radiosensitivity of the colony-forming units (CFU) differed depending on the site (the spleen, the bone marrow) of their colony formation. The CFU settling in the marrow proved to be more radioresistant (D(0) equalled 160-200 P) in comparison with the CFU settling in the spleen (D(0) constituted 80-100 P). It is supposed that a different radiosensitivity of the CFU was caused by the presence of heterogenic population of the stem cells and also by specific peculiarities of the organ (the spleen, the bone marrow) in which the colonies formed.
2494. [Submicroscopic identification of assumed hematopoietic stem cells (electron microscopic study of bone marrow cells with increased colony-forming capacity following the administration of oxyurea to mice].
作者: Z A Butenko.;K P Zak.;E V Klochko.;M A Baranovskiĭ.
来源: Probl Gematol Pereliv Krovi. 1975年20卷3期44-8页 2495. [Mathematical modeling of the dynamics of postradiation recovery of hematopoietic tissue].2496. [Model of regulation of the proliferation rate of bone marrow stem cells].
A mathematical model of speed regulation of the reproduction of bone marrow stem cells has been constructed and investigated, Two possible regulation mechanisms of proliferative activity of stem cells have been compared: at tissue level and at subtissual units level. The comparison of calculation results and experimental data has shown that the latter regultaion mechanism of stem cells proliferation would take place.
2497. [The effect of polysaccharide from typhoid bacteria on hemopoietic stem cells].
A study was made of dynamics of the content of colony-forming units (CFU) in the spleen and the bone marrow of the nonirradiated F1 (CBA times C57BL) mice to which polysaccharide (PS) from typhoid bacilli was administered. A method of exogenous colony formation was used in this work. A single PS administration increased the CFU count in the bone marrow (2-2 1/2 times) and in the spleen (3 times). Repeated (6- and 9- time) administration of PS was not more effective than a single one. Apparently PS acted as an inductor which caused proliferation of the stem hemopoietic cells and maintained this process for a definite period of time automatically.
2498. [Effect of the magnitude of the dose of Co60 gamma-rays on mouse bone marrow colony-forming cells].2499. [Determination of the number and radiosensitivity of bone marrow cells forming spleen colonies of erythroid, myeloid and megakaryocytic types].2500. [Effect of thymocytes on the quantity of polypotent hematopoietic mast cells in the spleen of sublethally irradiated mice].
With the aid of Till and McCulloch's method, 5 times 10(-6) thymic cells were found to cause an increase in the number of hemopoietic endogenous spleen colonies in syngeneic donor-recipient combination. Thymic cells of C57BL mice had no effect on the number of endogenous colonies in the spleen. 40 times 10(-6) thymic cells administered 24 hr after sublethal irradiation caused an increase in the number of colony forming units in the spleen within 14 days. Possible ways of the thymus effect on hemopoiesis are discussed.
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