It has been shown that the extent of methylation of cytocine in DNA is inversely correlated with gene expression in many cases. The DNAs extracted from placental tissue, hydatidiform mole and choriocarcinoma tissue were examined to see whether the extent of cytocine methylation is correlated to the gene expression of placental peptide hormones and the transformation of trophoblast. First of all we measured the amount of methylated cytocine per total DNA with HPLC. We then examined cytocine methylation in hCG alpha,beta and hPL genes using methylation sensitive (Hha I, Hpa II) and non sensitive (Msp I) restriction enzymes and molecular hybridization. During pregnancy the total amount of methylated cytocine measured with HPLC increases gradually from 0.72 mol.% at first trimester to 0.92 mol.% at term. The DNA of WBC showed a higher level of methylated cytocine than placental DNA. The extent of DNA methylation in the peptide hormone genes increases during placental development. Hypomethylation in the hCG alpha gene was also seen in molar tissue which expresses a high amount of hCG. Therefore it is inversely correlated that gene expression of hCG alpha,beta and the extent of DNA cytocine methylation. Furthermore some restriction polymorphisms were observed with Msp I in hCG alpha and hPL genes which might be related to malignant transformation of the trophoblast.

We have encountered very unique cases which involve identical twin sisters who underwent surgical treatment for a unilateral breast cancer at the same age of 28. Each, after 13 years, developed cancer in the opposite breast. In addition yet another sister, the youngest, had to undergo the same surgical procedure, at the same age that her identical twin sisters had had their first operations. These findings may indicate the existence of factors which decide the age of the malignancy onset, in addition to the existence of a predisposing factor for carcinoma in the genes of breast cancer patients.