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1261. [Regulation of glial fibrillary acidic protein gene by scorpion venom against epileptic seizures].1262. [Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal].
作者: Kui-De Yin.;Xing-Mei Zhang.;Shi-Qiang Liu.;Le-Gong Li.
来源: Sheng Wu Gong Cheng Xue Bao. 2002年18卷4期468-71页
Chilling-sensitive rice varieties acquire chilling tolerance when their roots are exposed to water stress for short time. Caffeine-sensitive calcium signal was involved in this procedure. By using total RNA differential display, a chilling induced cDNA(ICT: induction of chilling treatment) was isolated from roots of chilling-sensitive rice variety. It was determined that it is a novel cDNA by homology searching. The transcript level of ict mRNA is up-regulated under chilling stress, it is decreased to low level when the samples were transferred to standard culture conditions. Pre-treated with mannitol for two hours is beneficial to inducing ICT level of expression. This chilling induction was inhibited by caffeine, suggesting that it may play a putative role in signal transduction of caffeine-sensitive calcium.
1263. [Experimental study of K562 cell apoptosis induced by harringtonine].
作者: Rong Li.;Xiao-li Liu.;Qing-feng Du.;Hong Tian.;Lan-lin Song.;Yan Zhang.;Yi Yang.;Shu-yun Zhou.
来源: Di Yi Jun Yi Da Xue Xue Bao. 2002年22卷9期788-90页
To elucidate the mechanism by which harringtonine (HT) induces apoptosis in K562 cell line.
1264. [Analysis with DNA chips of the changes of gene expressions in K562 cells in response to As2O3 treatment].
作者: Chun-qiong Feng.;Wen-li Ma.;Ling Li.;Yan-bin Song.;Rong Shi.;Qing-hua Wu.;Qiu-ye Guo.;Ji Zhu.;Wen-ling Zheng.
来源: Di Yi Jun Yi Da Xue Xue Bao. 2002年22卷9期772-5页
To investigate differential gene expression in apoptotic cells induced by As(2)O(3), and identify novel apoptosis-related genes.
1265. [Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy].
作者: Ge Wang.;Xiao Rong Zhang.;Lu Hu.;Jun Wang.;En Ren Leng.;Dian Chun Fang.;Xiao Ming Yang.;Yong Zhang.;Fu Chu He.
来源: Zhonghua Gan Zang Bing Za Zhi. 2002年10卷4期256-9页
To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatectomy on rapidly induced expression of immediate early gene.
1266. [The development of peptide nucleic acid in gene regulation].
Peptide nucleic acid(PNA) is a kind of artificial DNA mimic. PNA hybridizes with DNA or RNA by means of Watson-Crick's base-pairs complementary with high stability, affinity and selectivity. PNA not only regulates. DNA replication, but also adjusts DNA transcription (or reverse transcription) and translation. Many applications have been explored as a new kind of molecular biological tool and a gene-targeting strategy.
1267. [Induction of sesquiterpene cyclase gene expression and antioxidant enzymes regulated by exongenous salicylic acid in leaves of Capsicum annuum].
An analysis of the induction of sesquitèrpene cyclase gene expression and antioxidant enzymes in Capsicum annuum by exongenous salicyclic acid (SA) pointed out that the sesquiterpene cyclase gene expression in detached pepper leaves was induced by 0.5-4 mmol.L-1 of exongenous SA, whereas the enzyme activity and accumulation of cyclase mRNA were much lower, and it took a longer time (36 h) for the gene to be induced to express after SA treatment, comparing to other elicitors such as UV and fungi treatment. The activities of SOD and POD were enhanced, while the CAT activity was inhibited to some degree. Consequently, H2O2 content was increased in SA treated pepper leaves. H2O2 accumulation was related to the integrated influences from antioxidant enzymes.
1268. [Effects of the combination of eicosapentaenoic acid and retinoic acid on the proliferation and differentiation of HL-60 cells].
To study the effect of combination of eicosapentaenoic acid (EPA) and retinoic acid (RA) on the proliferation and differentiation of HL-60 cells and its mechanisms.
1269. [The role of transforming growth factor-beta(1) in smoking-induced chronic bronchitis and emphysema in hamsters].
作者: Li Li.;Yingmao Ruan.;Ying Chen.;Yan Chu.;Xinlin Xu.
来源: Zhonghua Jie He He Hu Xi Za Zhi. 2002年25卷5期284-6页
To study the role of transforming growth factor-beta(1) (TGF-beta(1)) in the pathogenesis of chronic bronchitis and emphysema.
1270. [The effect of triptolide on the proliferation of airway smooth muscle and the expression of c-fos and c-jun in asthmatic rats].
作者: Yuanli Zhang.;Jianyou He.;Biao Liang.;Zeqing Song.
来源: Zhonghua Jie He He Hu Xi Za Zhi. 2002年25卷5期280-3页
To study the effect of triptolide on airway smooth muscle (ASM) proliferation and expressions of c-fos and c-jun in asthmatic rats.
1271. [Nitric oxide modulates expression of matrix metalloproteinase in asthmatic rats].
To observe the effect of nitric oxide on expression of matrix metalloproteinase and tissue inhibitor of metalloproteinase in asthmatic rats.
1272. [Losartan in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of monocyte chemoattractant protein-1 and basic fibroblast growth factor].
作者: Xiangqun Fang.;Yuanjue Zhu.;Xiaoling Hu.;Yongjian Liu.
来源: Zhonghua Jie He He Hu Xi Za Zhi. 2002年25卷5期268-72页
To study the effect of losartan on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.
1273. [Regulated expression of insulin gene in non-beta cell].
作者: Kuntang Shen.;Xinyu Qin.;Xin Zhang.;Zhihong Cheng.;Xiangru Xu.;Zeguang Han.
来源: Zhonghua Yi Xue Za Zhi. 2002年82卷11期780-3页
To engineer 293 cells to an artificial beta cell line which secrets insulin in response to stimulation by doxycycline.
1274. [Regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma by estrogen and insulin-like growth factor-1].
作者: Xiaohong Zhang.;Lihui Wei.;Jianliu Wang.;Zheng Tu.
来源: Zhonghua Yi Xue Za Zhi. 2002年82卷12期836-9页
To investigate the regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma by estrogen and insulin-like growth factor-1 (IGF-I) so as to provide theoretical basis for clinical hormone treatment of uterine endothelium cancer.
1275. [Differentially expressed genes in vascular endothelial cell line ECV 304 induced by high density lipoprotein].
作者: Wencheng Zhang.;Baosheng Chen.;Wuwei Zeng.;Ling Bai.;Hong Xue.;Gang Wu.
来源: Zhonghua Yi Xue Za Zhi. 2002年82卷13期921-3页
To isolate and clone the differentially expressed genes in vascular endothelial cell induced by high density lipoprotein (HDL) so as to study the anti- atherogenesis molecular mechanism of HDL.
1276. [Effect of compound 861 on tissue inhibitor of metalloprotenase 1 gene expression of HSC-T6 cells].
作者: Chenghong Yin.;Hong Ma.;Aimin Wang.;Xuemei Ma.;Jidong Jia.;Baoen Wang.
来源: Zhonghua Gan Zang Bing Za Zhi. 2002年10卷3期197-9页
To observe the in vitro effect of compound 861 (Cpd 861) on tissue inhibitor of metalloprotenase 1 (TIMP1) mRNA levels of HSC-T6 cell.
1277. [Study on gene expression changes of arsenic sulfide treated NB4 cells].
作者: Chunhong Gu.;Fangyuan Chen.;Jieying Han.;Ye Teng.;Nianxian Shao.;Renrong Ouyang.
来源: Zhonghua Xue Ye Xue Za Zhi. 2002年23卷1期16-8页
To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
1278. [Study of the effects of quercetin on PML gene and protein expression and localization in leukemia cells].
作者: Lu Zhong.;Fangyuan Chen.;Jieying Han.;Nianxian Shao.;Renrong Ouyang.
来源: Zhonghua Xue Ye Xue Za Zhi. 2002年23卷2期87-90页
To investigate PML gene and protein expression and localization in leukemia cell lines.
1279. [Reversal of nomegestrol acetate on multidrug resistance in drug-resistant human breast cancer cell line MCF7/ADR].
作者: Jie Li.;Liangzhong Xu.;Kailing He.;Weijian Guo.;Xiongzeng Zhu.;Yunhong Zheng.;Peng Xia.
来源: Zhonghua Zhong Liu Za Zhi. 2002年24卷2期129-32页
To study the reversal effect of nomegestrol acetate (NOM) on mutidrug resistance (MDR) in MCF7/ADR and its mechanism.
1280. [Up regulation of phenylacetate to glioma homeobox gene expression].
Even though phenylacetate (PA) bas been shown to inhibit the growth and induce differentiation in rat C6 glioma cell line, its mechanisms are still poorly understood. This study is aimed to identify which Hox gene is related to glioma and to observe the change in expression on mRNA level as treated by phenylasetate.
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