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1061. [Regulation of AAV-mediated glial cell-line derived neurotrophic factor expression by using improved Tet-on trans-activator].
作者: Teng Chen.;Xin-Gang Li.;Cheng-Yuan Wu.;Okada Takashi.;Ozawa Keiya.
来源: Zhonghua Yi Xue Za Zhi. 2005年85卷6期405-8页
To control the expression of AAV-mediated glial cell-line derived neurotrophic factor (GDNF) gene purposely by incorporating novel Tet-On trans-activator rtTA2s-S2, which prevents potential harms caused by over-expression of recombinant target genes.
1062. [A study of interferon-gamma induced airway mucous cell apoptosis and its mechanisms].
作者: Zhao-quan Shi.;Yan Feng.;Yuan-kai Hou.;Tao Liu.;Qing-yu Xiu.
来源: Zhonghua Jie He He Hu Xi Za Zhi. 2005年28卷3期160-3页
To investigate the effect of interferon-gamma (IFN-gamma) on airway mucous cells and its mechanisms.
1063. [Sensitivity of survivin-positive primary leukemia cells to chemotherapy].
To explore the sensitivity of survivin-positive primary acute leukemia (AL) cells to various chemotherapeutics, RT-PCR was used to detect the expression of survivin mRNA in AL cells, the cytotoxicity of chemotherapeutics was investigated with the MTT assay. The results indicated that for the primary AL cells cultured with various chemotherapeutics for 15 hours, the cytotoxicities of daunorubicin (DNR), homoharringtonine (HHT), aclacinomycin (Acla), Ara C (cytosine arabinoside), HA (HHT + Ara C), DA (DNR+Ara C) to the survivin-positive AL cells were similar with that to the survivin-negative AL cells. When AL cells were treated with AA (Acla + Ara C) in vitro, the cytotoxicity in survivin-positive group was apparently higher than that in survivin-negative group [(37.24 +/- 18.36)% vs (24.32 +/- 9.33)%, P = 0.032]. It is suggested that the survivin-positive AL cells may be sensitive to some chemotherapy, such as AA (Acla + Ara C).
1064. [Analysis of the methylation in the promoter of LRP15 gene and its expression].
作者: Zhou-Min Xu.;Li Yu.;Fang-Ding Lou.;Xue-Chun Lu.;Li-Ping Dou.;Long Yang.;Yan Chen.;Ming Lü.;Jie Cui.
来源: Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005年13卷2期188-91页
To study the methylation in the promoter of LRP15 gene and its relationship with gene expression and to explore the possible mechanism of regulating LRP15 gene methylation, the methylation in the promoter of LRP15 gene in K562 cell line was detected by MS-PCR. Then K562 was exposed to 5-aza-2'-deoxycytidine (CdR) and trichostatin (TSA), to determine whether the silencing of LRP15 gene by de novo methylation could be reversed. As a result, it was confirmed by MS-PCR that the promoter of LRP15 was hypermathylated in K562 cell line, and lost its transcription activity. After CdR, with or without TSA, the silencing of LRP15 gene by de novo methylation can be reversed. Observation demonstrated that the expression of LRP15 was controlled by methylation in its promoter in K562. It is suggested that methyltransferase inhibitor and deacetylase inhibitor may be effective agents in leukemia therapy.
1065. [Downregulation of transforming growth factor-beta1 and platelet-derived growth factor gene expression by interleukin-10 in murine hepatic stellate cells in response to experimental liver injury].
作者: Liang-fa Xiong.;Xi-sheng Leng.;Yu-hua Wei.;Tao Li.;Yan-tong Guo.;Zhi-zhong Qing.;Ji-run Peng.
来源: Zhonghua Wai Ke Za Zhi. 2005年43卷5期282-6页
To investigate the effect of interleukin-10 (IL-10) on the expression of transforming growth factor-beta(1) (TGFbeta(1)) and platelet-derived growth factor (PDGF) in hepatic stellate cells (HSC) during liver injury.
1066. [Expression of tobacco SKP1 gene induced by oligochitosan].
作者: Fu-Yun Zhang.;Bin Feng.;Yu-Guang Du.;Xue-Fang Bai.;Yu-Kui Zhang.
来源: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2005年31卷2期213-6页
Oligochitosan is an effective inductor for plant resistance. To understand the induced resistance mechanism, mRNA differential display was used to isolate genes from Nicotiana tabacum var. Samsun NN and four enhanced-expression gene fragments were obtained and were reamplified. The reamplified products of appropriate size were isolated and purified before they were subcloned into PMD18-T vector. The results of plasmids digestion by EcoRI and HindIII and analysis of reverse Northern blot indicated that the expression of the four genes was enhanced by oligochitosan induction. Sequence and homology analysis show that they share 82% identity in nucleotide sequence with Nicotiana benthamiana SKP1 gene. Because the SKP1 protein as the core component of SCF (ubiquitin ligase enzyme E3) is relevant to plant resistance to virus, so these results suggested that oligochitosan can induce plant resistance and its mechanism may be relevant to ubiquitination.
1067. [Changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae Rhamnoides L].
Hippophae rhamnoides L. possesses functions of antioxidation and radioprotection. This study was designed to investigate changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae rhamnoides L. (FHR) with cDNA microarray, and to explore possible mechanism of signal transduction on apoptosis.
1068. [Regulatory effect of STAT5 decoy oligonucleotides on trans-activation of bcl-x gene promoter in K562 cells].
作者: Jian-Ming Zeng.;Wen-Li Feng.;Xiao-Zhong Wang.;Mei Shi.;Zhi-Guang Tu.;Zong-Gan Huang.
来源: Ai Zheng. 2005年24卷4期414-8页
Constitutive activation of signal transducers and activators of transcription 5 (STAT5) plays an important role in malignant transformation of chronic myeloid leukemia (CML) cells. This study was to explore regulatory effect of STAT5 decoy oligonucleotides (ODNs) on trans-activation of its downstream target bcl-x gene in K562 cells.
1069. [Fluorosis on expression of nicotinic acetylcholine receptors in protein and gene levels in human SH-SY5Y neuroblastoma cells].
作者: Zhi-zhong Guan.;Ke-ren Shan.;Jin Xiu.;Yi-guo Long.
来源: Zhonghua Yu Fang Yi Xue Za Zhi. 2005年39卷1期26-9页
To investigate the influence of fluorosis on nicotinic acetylcholine receptors (nAChRs) in protein and gene levels in SH-SY5Y cells and the mechanism of the receptor modification.
1070. [In silicon cloning of HV126, a novel human gene related to multi drug resistance in leukemia].
作者: Jinrong Fu.;Wenli Liu.;Jianfeng Zhou.;Yufeng Zhou.;Youhua Xu.;Xianqing Jin.
来源: Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005年22卷2期158-63页
To find the novel gene related to the multi-drug resistance in leukemia and explore the molecular mechanism of multi-drug resistance.
1071. [Function of auxin-binding protein gene during cucumber fruit development].
作者: Ji Gang Bai.;Xiu Juan Wang.;Qian Xun Yin.;Yu Zhen Xu.;Rui Xue Zhao.
来源: Shi Yan Sheng Wu Xue Bao. 2004年37卷6期494-500页
A cDNA fragment that encodes auxin-binding protein 1 was amplified by, reverse polymerase chain reaction from ovary of cucumber. Its expression signals were weak in the ovary of 1 d before anthesis, while got strong in 2, 4 and 6 d after pollination. Among the unpollinated ovary of 2 d after anthesis, those that got enlarged had strong expression signals; the others that were wilting had weak signals. This indicated that auxin-binding protein 1 gene possibly play a role in cucumber fruit development. When auxin-binding protein 1 gene of Arabidopsis was transformed into cucumber, the parthenocarpic rate of transgenic plants was 31.7%, higher than the control. This result showed that the sensibility to auxin was increased in transgenic plants.
1072. [The effect of 20(R)-ginsenoside Rg3 on the differential expression of cell signaling genes and other related genes in human lung adenocarcinoma cell line A549].
作者: Ming-wei Chen.;Lan Yang.;Lei Ni.;Xuan Guo.;Rong-zhe Tian.;Xiao-qin Wang.
来源: Zhonghua Jie He He Hu Xi Za Zhi. 2005年28卷1期37-40页
To study the effect of 20(R)-ginsenoside Rg3 [20(R)-Rg3] on the differential expression of cell signaling genes and other related genes in human lung adenocarcinoma cell line A549.
1073. [Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs].
作者: Yu-ying Ma.;Xin-min Zhang.;Shi-feng Li.;Yi-feng Gu.;Wei-hua Chen.
来源: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005年25卷2期138-42页
To explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.
1074. [The inhibitory effect of deoxynivalenol on TAP-1 expression in human peripheral blood mononuclear cells in vitro].
作者: Yue-hong Li.;Xiang-hong Zhang.;Ling-xiao Xing.;Xia Yan.;Jun-ling Wang.;Feng-rong Wang.
来源: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005年21卷2期246-8页
To explore the effects of deoxynivalenol (DON) on transporter associated with antigen processing-1 (TAP-1) expression in human peripheral blood mononuclear cells (PBMCs).
1075. [Construction of recombinant deltaN IkappaBalpha adenovirus and its suppression of NF-kappaB activity in A549 cells].
作者: Zi-lu Zhu.;Zhi-xiang Cheng.;Jun Xiong.;Bing-ying Chen.
来源: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005年21卷2期171-4页
To explore the regulatory effect of deltaN IkappaBalpha gene on the activity of nuclear factor-kappaB(NF-kappaB).
1076. [Expression of CD14 protein in U937 cells induced by vitamin D3].
作者: Hang Sun.;Chuan-xin Wu.;Jian-ping Gong.;Hai-zhong Liu.;Xu-hong Li.;Hai-bo You.;Xian-feng Chen.
来源: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005年21卷2期155-8页
To observe the 1, 25-dihydroxy vitamin D3 (Vit D3) induced CD14 expression in human U937 cell line and the reaction of the cells to LPS stimulation following the induction.
1077. [Effect of tetrandrine combined with Droloxifen on the expression of bcr/abl of K562 at both mRNA and protein levels].
作者: Bao-An Chen.;Xi-Jun Qian.;Jian Cheng.;Feng Gao.
来源: Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005年13卷1期95-9页
To observe the effect of Tetrandrine (tet) combined with Droloxifen (DRL) on the expression of bcr/abl mRNA and P(210) BCR/ABL protein of K562 cell line, after K562 cells were cultured in the medium containing Tet (1 micromol/L), DRL (5 micromol/L) separately or in their combination for some time, the changes of bcr/abl mRNA and protein expression were detected by RT-PCR and Western blot respectively. The results showed that the application of single drug of Tet or DRL had no effect on bcr/abl mRNA and BCR/ABL protein expression in K562 cell line. However, Tet in combination with DRL began to downregulate bcr/abl mRNA and P(210) BCR/ABL expression of K562 cells at 48 h and 72 h, respectively. It is concluded that tetrandrine in combination with Droloxifen can downregulate the expression of bcr/abl mRNA and P(210) BCR/ABL protein and the combination may be involved in the mechanism underlying the reverse effects on multidrug resistance in leukemia.
1078. [Proliferation inhibition effect of indomethacin on CML cells associated with down-regulation of phosphorylated STAT1/STAT5 and inhibition of COX-2 expression].
To explore the mechanism of anti-proliferative effect of indomethacin (IN) on chronic myelogenous leukemia (CML) cells.
1079. [pCKM-mPTH recombinant plasmid therapy for hypoparathyroidism: an in vivo experimental research with rats].
To investigate the effect of gene therapy with pCKM-mPTH recombinant plasmid on hypoparathyroidism.
1080. [Gene expression differences between the anti-BPDE-transformed and normal cells by suppression subtractive hybridization].
作者: Shejuan An.;Jiakun Chen.;Xuemin Chen.;Yanfeng Zhao.;Lili Liu.
来源: Wei Sheng Yan Jiu. 2004年33卷6期651-3页
To screen the gene expression differences between the BPDE-transformed and normal cells.
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