To build a new rat brain glioma model with LacZ as a reporter gene.

To study the expression of nm23-H1 gene in endometrial carcinoma and its relationships between the clinicopathological parameter and prognosis of endometrial carcinoma.

To investigate the relationship among DNA content, cell cycle phase analysis with the clinical-pathological features and prognosis of uterine sarcomas.

To find a new method to molecular biological detection of lung cancer.

To study the effects of exogenous wild type p53 suppressor gene on malignant growths of human lung cancer cell line with mutant type p53 gene.

Evaluate the clinical potential of HSV-TK/GCV system in gene-therapy of tumors by using this system to treat human lung cancer A549 cells in vitro and in vivo.

To study the inhibitory effects of retro-virus-mediated p16 gene on human ovarian cancer cell line CAOV3.

In order to investigate the suppression effect of tumor suppressor genes in lung adenocarcinoma.

To investigate the polymorphisms of CYP1A1 and GSTM1 genes as well as their separate and combined effects on individual susceptibility to lung cancer.

To investigate the mechanism by which TGF beta 1 inhibits the growth of leukemic cells and the significance of serum TGF beta 1 level in acute leukemia(AL) patients.

To elucidate the relationship between bcl-2 and hemangioma.

To study the biological characters of 137 cases of surgically treated NSCLC and their correlation with stages, types and survival rates.

To study alteration of several genes in the process of primary lung cancer.

To investigate the expressions of C-erbB2, nm23 and p53 in epithelial ovarian cancer and their clinical significance.

Using citric-LSAB-microwave immunohistochemical technique, the expression of c-myc was studied in different regions of larynx including laryngeal squamous cell carcinoma (LSCC), border area, adjacent mucosa which was 0.5, 1.0, 1.5 and 2.0 cm away from cancer 30 cases and four cases of normal laryngeal mucosa. The results showed that over-expression of c-myc in LSCC was found in 29 of 30 (96.7%), which was higher than that in normal laryngeal mucosa (P < 0.01). A significant different expression of c-myc can be seen at the regions of 2.0 and 1.5 cm distant from the tumor (P < 0.05). The expression of c-myc increased as tissue progressed in sequence from normal mucosa, adjacent mucosa to carcinoma. The c-myc expression did not correlate with site of tumor, stage and histological grade (P > 0.05). The results indicate that over-expression of c-myc is involved in genesis of LSCC, and may be an early event in the development of human squamous-cell carcinoma of the larynx. The tissue adjacent to the tumor may have a potential malignancy.

In order to study the relationship between bcl-2 gene and nasopharyngeal carcinoma. The two hot breakpoints of bcl-2 gene and bcl-2 protein expression were examined by in situ hybridization and immunohistochemistry technique in 41 nasopharyngeal carcinoma(NPC) tissues. bcl-2 gene breakpoint were found in 4 cases of NPC(positive rate 9.8%). 34 cases of NPC expressed bcl-2 protein, the positive incidence was 82.9%. No statistically significant differences was found in histological grades. All benign lesions were negative for bcl-2 gene breaking and bcl-2 protein expression. There was no corresponding relation between bcl-2 protein expression and bcl-2 gene breaking. The results implicated that bcl-2 gene breaking may not play an important role in the pathogenesis of NPC. It also suggested that overexpression of bcl-2 protein may involved in carcinogenesis of nasopharyngeal epithelium.

To investigate the expression of p16 gene in non-Hodgkin's lymphoma (NHL) and its clinicopathological significance.