Semipermeable membrane devices (SPMDs) in combination with in vitro ethoxyresorufin-O-deethylase (EROD) assay was used to assess the aryl hydrocarbon receptor (AhR) effects in aquatic system. In present work, polycyclic aromatic hydrocarbons (PAHs) were selected as target compounds and SPMDs were deployed simultaneously with caged crucian carp for 32 days at five sites in Meiliang Bay, Taihu Lake. The concentrations of PAHs in the SPMD dialysates and fish tissues were analyzed by gas chromatography-mass spectrometry. In addition, the EROD activity of SPMD dialysates was assayed with H4IIE rat hepatoma cells and the induction of EROD activity in pancreas and liver of fish was also assayed. It was found that dialysates from SPMDs with longer exposures generally induced stronger activity and the toxic potency of dialysates expressed as bioassay-derived 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents ranged from 3.8 pg/g to 6.2 pg/g for 32 days exposure. A correlation of toxicity equivalency quantity (TEQ) with chemical analysis and in vitro bioassay-derived TEQ(bio) of SPMD dialysates yielded an R2 of 0.88, therefore, indicated that PAHs were one of the important AhR agonists in the water. Empirical evidence suggests that PAHs can account for about 40% to 50% of the potency observed. Moreover, a good correlation was observed between the results of EROD bioassay in vivo and EROD bioassay in vitro (R2 = 0.62), thus reflected that these two methods can be complementarily each other. It is, therefore, suggested that the SPMD technique combined with chemical analyses and in vitro EROD assay might be a valuable monitoring tool to assess the levels and effects of AhR agonists in water ecosystem.

To investigate the effects of sex hormones on sarcoplasmic reticulum (SR) Ca(2+)-ATPase activity and gene expression in the genioglossal muscle of the aged rats, and to explore the possible mechanism related to the upper airway stability.

To investigate the effects of dexamethasone (DEX) on the expression of intercellular adhesion molecule-1 (ICAM-1) in orbital fibroblasts (OF) from patients with thyroid associated ophthalmopathy (TAO).

To evaluate the effects of recombined human Neuregulin on the contractibility of cardiac muscles of Rhesus Monkeys with pacing-induced heart failure and to reveal the possible mechanisms involved.

To investigate the sensitivity to Adriamycin of down-regulated cells expressed hOGG1 gene which target to oxidative DNA damage and repair, and provide more experimental evidence of sensitizing the response of tumor to chemotherapy.

To study the effect of curcumin on the apoptosis of prostate cancer cell line LNCaP and regulation of expression of maspin gene.

To observe the effect of atorvastatin on left ventricular remodeling in spontaneously hypertensive rats (SHR), and to explore its possible mechanism involved.

To investigate the effect of different stimuli and different culture conditions on the cytokine expression of CD4+ and CD8+ T cells.

To evaluate the effect of aldosterone (ALD) and spironolactone (SPI, one of the aldosterone receptor antagonists) on synthesis and secretion of fibronectin (FN) and expression of transforming growth factor-beta1 (TGF-beta1) mRNA in cultured rat mesangial cells.

To study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups on proliferation and c-myc gene expression of SGC-7901 cells in vitro.

To study the effect of resveratrol to the onset and progress of acute lung injury in rat model of severe acute pancreatitis (SAP).

To investigate the molecular mechanism of osteogenetic differentiation of bone-marrow mesenchymal stem cells (BMSCs) and the probability using the BMSCs to gene therapy for bone fractures.

To evaluate the effect of PKC-delta inhibitor Rottlerin on human colon cancer cells and its mechanism.

To investigate the effects of qiongyugao on the expression of hepatitis B x antigen (HBxAg) in BALB/c-nu mice into which human hepatic carcinoma cells were transplanted, and to analyze its specific mechanism in prophylaxis and treatment of hepatocellular carcinoma.

To study the antipyretic effect and mechanism of Daqingye injection (DQYI).

With root injection and foliar spray, this paper studied the effects of different concentrations salicylic acid, brassinolide, chitosan and spermidine on the growth, morphogenesis, and physiological and biochemical characters of cucumber ( Cucumis sativus L. ) seedlings under 200 mmol x L(-1) NaCl stress. The results showed that at proper concentrations, these four exogenous chemical activators could markedly decrease the salt stress index and mortality of cucumber seedlings, and the decrement induced by 0. 01 mg x L (-1) brassinolide was the largest, being 63. 0% and 75. 0% , respectively. The activities of superoxide dismutase (SOD) , peroxidase (POD) and catalase (CAT) increased significantly, resulting in a marked decrease of malondialdehyde (MDA) content and electrolyte leakage. The dry weight water content and morphogenesis of cucumber seedlings improved, and the stem diameter, leaf number, and healthy index increased significantly. All of these suggested that exogenous chemical activators at proper concentrations could induce the salt resistance of cucumber, and mitigate the damage degree of salt stress. The salt resistance effect of test exogenous chemical activators decreased in the sequence of 0.005 -0.05 mg (L-1) brassinolide, 150 -250 mg x L (-1) spermidine, 100 -200 mg x L(-1) chitosan, and 50 -150 mg x L(-1) salicylic acid.

To construct the model of non-steroidal anti-inflammatory drug (NSAID) induced gastropathy and observe the preventive effects of Teprenone on it in rats.

To investigate the association of S100A2 expression with the secondary radioresistance in pancreatic cancer cells.

To study the biological effects of ligation of CD40 mediated by soluble recombinant human CD40 ligand (srhCD40L) on the human malignant hematogenous cells and to explore the molecular mechanism thereof.

Expression strain of des-pGlu1-brazzein was constructed and the conditions using lactose as inducer was also optimized. The Influences of three factors which were lactose concentration, induction time and inducing temperature on the growth of strain and on the yield of des-pGlul-Brazzein was analyzed in detail. The result indicated that high lactose concentration inhibit the growth of strains (P < 0.01) but made no difference on expression of target protein between 0.5%-5% (P > 0.05), Biomass would be improved as time passed (P < 0.01), but the yield of target protein didn't increase obviously at 30 degrees C compared with at 37 degrees C. Further result showed that the greater expressed level of des-pGlul-Brazzein, as high as about 20% of total cell protein, could be achieved after the strain had been induced with 0.5% lactose under 28 degrees C - 30 degrees C for 4 h.