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7041. [Study of the multidrug gene transfer to bone narrow hematopoietic cells and clinical uses].7042. [Role of fibonectin in hematopoiesis].7043. [Treatment of malignant hematological diseases with autologous peripheral blood stem cell transplantation].
To observe the effectiveness of autologous peripheral blood stem cell transplantation(ABSCT) in the treatment of malignant hematologic diseases.
7044. [Studies on the committed differentiation of CD34+ hematopoietic progenitor cells].
To elucidate the capacity of different hematopoietic growth factors (HGFs) combinations for inducing CD34+ cells to proliferate and differentiate committedly to granulocytes, erythrocytes, megakaryocytes and dendritic cells (DC).
7045. [Analysis of surface markers of long-term cultured human umbilical cord blood hemopoietic cells].
To explore the optimal time for in vitro expansion and transplantation of umbilical cord blood hemopoietic cells.
7046. [Differentiation of embryonic stem cells into neuronal cells in vitro].
To investigate the probabilities of embryonic stem (ES) cells differentiating into neuronal cells in vitro.
7047. [Selected CD34+ cells transplantation: a primary clinical report].
CD34+ is an immunophenotype of hematopoietic stem cells/progenitors. CD34+ cells selection in vitro may deplete T-cells 4-5 logs and tumor cells 3-4 logs. It will benefit to mismatched related donor allo-transplantation and autologous transplantation of tumor diseases.
7048. [Variable number of tandem repeats as an evidence of engraftment status after allogeneic peripheral blood stem cell transplantation].
To evaluate engraftment status of patients after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) based on red blood cell type, karyotype and variable number of tandem repeats (VNTR).
7049. [The ex vivo expansion of cord blood hematopoietic cells: 1. Cell growth behavior].
The population growth and metabolism of hematopoietic cells from cord blood were investigated without and with hematopoietic growth factors and the long-term growth kinetics of cells was studied. In static cultivation of hematopoietic cells, the population specific growth rate was 0.34 d-1, and the average double time 2 days. The greater part of glucose was consumed and 40 mmol/L lactate was formed during cell culture. For the long-term culture, the maximum output of CFU-GM appeared between the 2nd and 3rd weeks and that of BFU-E in the first week. With 50% medium exchange, the expansions of total cells, CFU-GM, and BFU-E came up to 14, 13, and 5 times, respectively.
7050. [Autologus hematopoietic stem cells purged in vitro in breast cancer patients].7051. [Mobilization of autologous peripheral blood stem cells by cyclophosphamide and recombinant human granulocyte colony-stimulating factor(rhG-CSF)].
To observe the effect of cyclophosphamide (CTX) and recombinant human granulocyte colony-stimulating factor(rhG-CSF, Filgrastim) on autologous peripheral blood stem cells (APBSC) mobilization.
7052. [The predictor for platelet recovery after PBSCT].
In order to find out a predictor more accurate, reliable and convenient for platelet recovery capacity after peripheral blood stem cell transplantation (PBSCT).
7053. [Nitric oxide and its modulation of hematopoiesis].7054. [Effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria].
To study the effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria (PNH).
7055. [Analysis of CD34+ cell amounts in normal and abnormal bone marrow cells of patients with paroxysmal nocturnal hemoglobinuria].
To explore the contributive role of CD34+ cell amount in pathogenesis of clonal dominance in paroxysmal nocturnal hemoglobinuria (PNH).
7056. [Induction of apoptosis of CD34+ cells by serum from two patients with severe aplastic anemia].
To explore the relationship between the hematopoietic inhibitor and apoptosis of CD34+ cells in patients with severe aplastic anemia (SAA).
7057. [Induction of hematopoietic cell apoptosis by the serum from immune-mediated aplastic anemia mice].
To explore the mechanism of the impairment of hematopoietic stem/progenitor cells in aplastic anemia(AA) mice.
7058. [In vivo inductive effect of hemin on the erythroid progenitor of the normal and aplastic anemia mice].
To investigate the in vivo effect of hemin on erythroid progenitor of the normal and aplastic anemia mice.
7059. [The study of biocompatibility of titanium in middle ear cave of guinea-pig].
In order to supply full objective basis about tympanoplasty with titanium, 30 middle ear caves of guinea-pigs were implanted titanium ring in accordance with different time groups. Both before and after titanium ring were implanted, the guinea-pigs were detected in biochemical criterion of blood, titanium content of the hair, the changing of auditory threshold, tissue structural of middle ear and inner ear by energy spectrometry, electro-physiology, microscopy and scanning electron microscopy. The results showed that function of hepatorenal and titanium content of hair haven't significant change between pre- and postimplantation of titanium ring. Only 10 days group, it appeared the rising of auditory threshold and inverting of cilia by ABR and scanning electron microscopy. In 10 days and 1 month groups, there were blood stain and inflammatory cells under mucosa. The results suggested that titanium is good biocompatibility and no toxic for organism and mucosa.
7060. [In vitro study on transduction of human O(6)-methylguanine-DNA-methyltransferase cDNA into human umbilical cord blood CD34(+) cells].
作者: J Wang.;X Xia.;Z Chen.;D Lu.;J Xue.;C Ruan.
来源: Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000年17卷6期395-8页
To explore human umbilical cord blood hematopoietic progenitor cells transduced with human O(6)-methylguanine-DNA-methyltransferase (MGMT) gene increase resistance to 1,3-Bis(2-Chloroethyl)-1-Nitrosourea(BCNU).
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