To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).

Streptomyces avermitilis NRRL8165 could serve as a good host for heterologous expression of antibiotics biosynthetic gene clusters. However, it needs to improve its conjugation frequency to accept large plasmids.

Salmonella species are important food-borne pathogens of human and animal. S. enterica serovar Enteritidis is the only serovar that routinely causes human infection through intact egg, the molecular basis of its ability to survive in egg is poorly understood. The importance of post-transcriptional regulation by small non-coding RNAs (sRNA) has recently been recognized. The sRNAs play diverse physiological roles in stress responses, regulation of metabolism, control of bacterial envelope composition and bacterial virulence. In this study, we studied regulatory function of salmonella sRNA sraB associated with survival ability of S. enterica serovar Enteritidis in egg albumen.

A hydroponic experiment was conducted to study the effects of applying 100% NO3- -N, 100% NH4+ -N, and 75% NO3- -N+25% NH4+ -N on the nitrogen metabolism and the nitrate reductase (NR) and glutamine synthetase (GS) gene expression of cherry tomato during its fruit development. Applying 75% NO3- -N+25% NH4+ -N slightly increased the single fruit mass, and increased the fruit NH4+ -N, total amino acid, and total N contents and N accumulation significantly, compared with applying 100% NO3- -N. In treatments 100% NO3- -N and 75% NO3- -N + 25% NH4+ -N, the fruit NR activity and its gene expression had no significant difference, but were higher than those in treatment 100% NH4+ -N. The fruit GS activity was significantly higher in treatment 75% NO3--N+25% NH4+ -N than in treatment 100% NO3- -N. In the three treatments, isozyme GS1 (Cytosolic type GS) and GS2 (Chloroplast type GS) expression was inconsistent with GS activity, suggesting that the effects of applied N on GS activity could be mainly reflected at posttranscriptional level.

To investigate phytoestrogenic effects of ferulic acid in ER-positive T47D and ER-negative MDA-MB231 cells in culture.

Src tyrosine kinase and matrix metalloproteinase play the pivotal roles in lung cancer invasion and metastasis. The aim of this study is to evaluate the effect of Src tyrosine kinase inhibition on secretion of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) by non-small cell lung cancer (NSCLC) cells.

To explore the inhibition effect of RNA interference on the ICP4 expression and DNA replication of herpes simplex virus type 2 (HSV2).

To explore the action mechanism of action of Qingyi Huaji Formula (QYHJ), whether its inhibition on the growth of the tumor is by way of down-regulating Ski expression.

Abundant evidence has suggested that neuroinflammation participates in the pathogenesis of Parkinson's disease (PD). The emerging evidence has supported that microglia may play key roles in the progressive neurodegeneration in PD and might be a promising therapeutic target. Ganoderma lucidum (GL), a traditional Chinese medicinal herb, has been shown potential neuroprotective effect in our clinical trials that lead us to speculate that it might possess potent anti-inflammatory and immunomodulating properties. To test this hypothesis, the present study investigated the potential neuroprotective effect of GL and underlying mechanism through inhibiting microglial activation using co-cultures of dopaminergic neurons and microglia. The cultures of microglia or MES23.5 cells alone or together were treated for 24 h with lipopolysaccharide (LPS, 0.25 μg/mL) as a positive control, GL extracts (50-400 μg/mL) or MES23.5 cell membrane fragments (150 μg/mL) were used in treatment groups. Microglia activation, microglia-derived harmful factors and [(3)H]dopamine ([(3)H]DA) uptake of MES23.5 cells were analyzed. The results showed that microglia were activated by LPS and MPP(+)-treated MES23.5 cell membrane fragments, respectively. Meanwhile, GL extracts significantly prevented the production of microglia-derived proinflammatory and cytotoxic factors, including nitric oxide, tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β), in a dose-dependent manner and down-regulated the TNF-α and IL-1β expressions on mRNA level. In addition, GL extracts antagonized the reduction of [(3)H]DA uptake induced by MPP(+) and microglial activation. In conclusion, these results suggest that GL may be a promising agent for the treatment of PD through anti-inflammation.

To compare the different gene expression profiles among Qingkailing components of BA (baicalin), JA (jasminoidin), CA (cholic acid) and CM (concha margaritiferausta) in regulating hippocampus ischemia related genes of mice.

Our aim was to enhance nisin production by overexpression of nisin immunity gene nisI in nisin-producing strains.

By observing the rats with PDTC after the intervention, myocardial ischemic reperfusion injury (MIRI) during apoptosis, NF-κB and iNOS expression. Analysis of NF-κB and iNOS expression in the rat heart ischemic-reperfusion injury, apoptosis in the process of regulation and its mechanism.

To explore the effect of staphylococcal enterotoxin A(SEA) on CD3(epsilon) chain expression on mononuclear cells in cord blood, which were stimulated by K562 cells.

To investigate the antiproliferation effect of cardamonin (CAR) and its possible mechanisms on human umbilical artery smooth muscle cells (HUASMCs) cultured in the mimicking insulin resistance (IR) medium.

To explore the regulation of Bub1 mRNA expression in endometrial carcinoma cells by estrogen and paclitaxel.

The aim of our work is to investigate the effects of overexpression of two acetyl-CoA synthase genes, ACS1 and ACS2, on the physiological functions of Saccharomyces cerevisiae.

To investigate the influence of di-(2-ethylexyl) phthalate (DEHP) on cell apoptosis and expression of Bcl-2 and bax in cultured human first trimester cytotrophoblasts.

Until now, there are still some questions that chemotherapy could block cancer immunologic escape or not. The aim of this study is to explore the mechanism of paclitaxel in the blocking of immunologic escape in 3LL bearing mice.

The spider venom may inspire new drugs to treat cancer. The aim of this study is to investigate the effects and mechanisms of spider venom on lung adenocarcinoma cell A549.

To study the modulatory effect of Sophora flaveacens and co-administration with Veratrum nigrum on the activity and mRNA expression of cytochrome P450 isoenzymes in rat liver.