With the same forage to the mice, three experiments were done. The control group took water and experimental group took 1% taurine. 1. After the mice were vaccinated with S180 sarcoma, every group was further divided into two groups, one treated with cytoxan (CTX), another one treated with nothing. The survival period, weight of the tumor and the tumor inhibition rate were investigated. The result shows: The survival period of the group fed with taurine was (35.75 +/- 23.7) days longer than that of the control group (18.7 +/- 5.6) days, and the tumor inhibition rate was 42.26%. The mice fed with taurine and treated with CTX survived to 61 days with no death, and no tumor could be seen grossly, so the tumor inhibition rate is 100%. The survival period and weight of the tumor were not different between the CTX treated and control groups. 2. Tumor cell membrane fluidity was observed after the mice were vaccinated with Erlish ascitic tumor. The result shows: in the taurine group, the fluorescence polarization (p) is 0.108 +/- 0.020, the viscosity (eta) is 0.618 +/- 0.140 and in the control group p is 0.139 +/- 0.20, eta is 0.874 +/- 0.150, respectively. Thus the cell membrane fluidity of the taurine group is also obviously better than that of the control group (P < 0.01). 3. The immune functions of the two groups were determined by carbon clearance test and capacity of serum hemolysin. The immune function of the taurine group is also obviously higher than that of control group (P < 0.01). All the results described above show:taurine is functioning for tumor inhibition, and has obvious synergic effect while treated with CTX as chemotherophy. It is considered that the tumor inhibit function of taurine may be related to its effects of immune enhancing and membrane profecting.

To study the effect of low-molecular-weight Rehmannia glutinosa polysaccharides (LRPS) on p53 gene expression.

Chitosan is a kind of biological material with good histocompatibility and gradual biodegradability in vivo. It has no toxicity or side-effect. For its gradual degradation, chitosan and adriamycin were mixed and formed drug delivery system (DDS). The release test of DDS and exudant of DDS in inhibiting OS-116 were examined in vitro. The results were as following: the DDS could release adriamycin in slow and stable way. The SO-116 inhidition rate of the exudant of the DDS on the 1st, 20th, 40th and 60th day was 58.11%, 36.48%, 24.32% and 21.62% respectively. It was concluded that the drug delivery system was a slow release system. It could maintain the concentration of adriamycin in a certain level. It was also suggested that the chitosan was a good carrier for slow release of chemotherapeutic drug in local therapy for postoperative treatment of bone tumor.

C57/BL6J mice were inoculated with Lewis lung cancer cells as an experimental model to study the effects of green tea on cancer prevention, inhibition of tumor growth and immune regulation in mice with tumor. Results showed that weight of thymus in C57/BL6J mice and its index declined, proportion of positive CD4 subgroup of T lymphocyte and ratio of CD4+, to CD8+ reduced, baseline chemilumi-nescence decreased in peripheral white blood cells, yeast zymosan stimulated chemiluminescence increased, and number of immunoglobulin M formation cells decreased. It indicated that green tea had obvious inhibition in Lewis lung cancer and protective effects, to various extent, on adverse changes of above indices.

To investigate the effect of C1027, an enediyne antitumor antibiotic, on angiogenesis and its anti-metastatic activity.

To study the relation between the PML-RAR alpha gene and the effects of ATRA on proliferation and differentiation in acute promyelocytic leukemia cell line NB4 cells.

60 cancer patients during chemotherapy were investigated by using case-control study. 30 of them were recruited as the experiment group and given biofeedback relaxation training, the others were controls. Both of the two groups were assessed with SCL-90 and questionnaire on physical reactions. Results showed that no significant difference was found between the average scores of SCL-90 (including F1, F3, F4, F5, F10) of the two groups before the intervention. However, lower average scores and less serious physical reactions (4 of the 6 items) were seen from the experiment group after the intervention. It is indicated that biofeedback relaxation training is effective in alleviating patient's unhealthy psychosomatic reactions during chemotherapy.

Recent studies indicate that wild-type p53 can trigger cell apoptosis induced by many chemotherapeutic agents which induce DNA damage or cause disruptions of DNA metabolism, such as ADM, 5-FU, VP-16 and radiation. We introduced the wild-type p53 gene into a MDR cell line KBV200 in which the endogenous p53 was found to be rearranged. By G418 selection and Northern blot analysis, a G418-resistant clone named KBV200-p53 was obtained which continuously expressed the exogenous wild-type p53 mRNA. After treatment with Vincristine(VCR), the wild type p53-expression cells presented typical morphology characteristic of apoptosis analysed under electron and fluorescence microscopes. Flow cytometer analysis showed that the KBV200-p53 cells were more readily undergo apoptosis than their parental cells KBV200. After treatment with VCR 600 nmol.L-1 for 24 h, the apoptotic percentage of KBV200-p53 and KBV200 cells was about 42.4% and 8.4%, respectively. This result indicates that wild-type p53 stimulates VCR-induced apoptosis in KBV200 cells.

To study the relationship between multi-drug-resistance (MDR1) gene expression and the drug resistance of ovarian carcinoma and the reversing potency of drug-resistance modifying agent--cyclosporin A (CsA).

To define the prevalence of acquired red cell enzymopathy in leukemia and MDS patients and explore its clinical significance.

The effects of differentiation of human leukemia HL60 cells on harringtonine(Har) and camptothecin(Cam) induced apoptosis(in these cells) were studied. When treated with phorbol 12-myriate, 13-acetate 16 nmol.L-1 for 24 h, the HL60 cells differentiated into monocyte/macrophage cells and were arrested at G1 phase. The differentiated cells were shown to be resistant to the Har and Cam induced apoptosis, but showed no change of expression of c-myc gene. HL60 cells incubated in 1.4% dimethyl sulfoxide for 48 h differentiated into granulocyte cells and were also gene arrested at G1 phase. The differentiated cells became resistant to the apoptosis induced by Cam, but not that by Har, and expression of c-myc decreased drastically in the differentiated cells. The results indicate that the differentiated status of human leukemia HL60 cells apparently affected the apoptosis induced by harringtonine and camptothecin, but it was irrelevant to the change of the expression of c-myc gene.

To examine the accumulation of anticancer drugs in tumor tissues by different routes of administration.

To analyse the clinical course and treatment result of lung metastases from breast cancer.

To evaluate the efficacy of hyperthermic-hypoosmotic solution alone and in combination with anti-tumor drugs in the growth inhibition of human gastric cancer xenografts in immuno-compromised Balb/c mice.

Establishment of cell apoptotic model of human hepatocellular carcinoma.

To evaluate whether the growth inhibition by retinoic acid and RAR alpha mRNA expression levels were affected by the change of ER expression.

To evaluate the effects of traditional Chinese medicine formulae (Bailong) on cAMP/PKA and diacylaglycerol (DAG) protein kinase (PKC) pathways of the MGc80-3 cells.

Cultured human gastric cancer cell line PAMC82 was studied in vitro to further verify anti-tumor effect sof rare-earth elements and explore their mechanism of tumor inhibition. Inhibitory effects of elements lanthanum and cerium on cell growth, reverse effects of them on reduction of malignancy and effects of them on level of expression of oncogene and cancer suppressor gene were observed. Lanthanum chloride, cerium chloride and mixed rare-earth chloride at levels of 0.5 to 1.5 mmol/L could inhibit obviously growth of cancer cells and change cell morphology and microtubule structure of PAMC82, similar to that of normal cells, their colony-forming ability lowered in soft agar, and expression of tumor suppressor gene p53, p16 and p21 increased and that of gene nm23 lowered.