To test the hypothesis that wild-type p53 regulates the expression of p-glycoprotein.

To explore the effect of tripterine on leukemic mast cells.

To explore the relationship among intercellular -SH, caspase, retinoic acid (RA) and arsenic trioxide(As2O3)-induced apoptosis.

To study the in vitro effect of cord blood plasma(CBP) on the sensitivity of acute myeloid leukemia(AML) cells to cytosine arabinoside (Ara-C).

To investigate the role of CDKs in the RA inducing HL-60 cell differentiation.

To study the electrochemical behavior of apoptotic HL-60 cells induced with etoposide.

To find more effective chemotherapeutic agents and treatment regimens, we studied the cytotoxicity of cisplatin to human laryngeal squamous cell carcinoma strain Hep2.

Antibiotic G0069A, produced by a Streptomyces strain isolated from a soil sample collected in Yunnan Province, China, has been verified as a clavam peptide. Determined by MTT assay, G0069A showed highly potent cytotoxicity to cancer cells with multidrug resistance. The IC50 values of G0069A to KB and KB/VCR cells were 0.60 and 0.46 mumol.L-1, and to MCF-7 and MCF-7/ADM cells were 1.4 and 1.2 mumol.L-1, respectively. G0069A displayed equally potent cytotoxicity to the parent cell lines and their resistant sublines. When administered by i.v. or i.p. route at tolerable doses, G0069A exhibited markedly inhibitory effect on the growth of sarcoma 180 and hepatoma 22 in mice. At dose level of 3 mg.kg-1, i.v., x3, sarcoma 180 and hepatoma 22 were suppressed by 87%(P < 0.01) and 72%(P < 0.01), respectively. The results indicate that G0069A is a beta-lactam antibiotic showing antitumor activity.

The total synthesis of cimicifugamide, a new natural compound isolated from the roots of Cimicifuga dahurica, was accomplished by a reaction sequence of seven steps in an overall yield of 31%. Trifluoroacetoxy was used as leaving group at the anomeric carbon. The target product was characterized by IR, MS, 1HNMR, 13CNMR and elemental analysis. In addition, seven analogues were synthesized and their preliminary pharmacological activities were tested.

A series of epipodophyllotoxin carboxylates were prepared from podophyllotoxin by reacting with organic acids under the catalysis of BF3.Et2O. All these products were characterized through IR, 1HNMR, MS and elemental analysis. These compounds showed significant antitumor activities against mouse leukemia P388 and human stomach cancer SGC-7901 in pharmacological tests in vitro.

The inhibiting activities of five antitumour drugs including "An ke su" to human cancer cell lines coming from 19 patients were measured in vitro with MTT assay, which can provide proper antitumor agents for clinical chemotherapy. The evaluation rate of medicine sensitivity is 89.47%, which is same as the result of clinical therapy. This method indicates the response of tumour cells to "An Ke Su" and other agents. Being simple, sensitive and rapid, it proves to be valuable in clinical therapy.

Endostatin is a newly found inhibitor of angiogenesis, which is identified as c-terminal 184 amino acid fragment of collagen XVIII NC1-domain. A 570 bp cDNA fragment of endostatin has been amplified by PCR from a commercial human fetal liver cDNA library. After subcloned into the yeast vector pPIC9 and subsequence to prove its correctness, Pichia pastoris was transformed with the recombinant pPIC9-endostatin. The expressed endostatin in P. pastoris was purified by heparin-sapherose affinity chromatography. It's purity identified by SDS-PAGE thin layer scanning analysis was up to 98.7% and its Mol. Weight measured by MS was 20.34 kD. The expression level was up to 40 mg/L. The first fifteen amino acid sequence of the N-terminal was completely identical with the inner sequence C-terminal fragment of collagen XVIII NC1 domain as has been designed. Bioassay indicated that the recombinant endostatin can inhibit angiogenesis stimulated by bFGF in CAM test and also the proliferation of both HUVEC and ECV304 in an in vitro test.

To study the effects of platelet factor 4(PF4) and tetrapeptide N-acetyl-Ser-Asp-Lys-Pro(AcSDKP) on hemopoietic progenitors in mice treated with 5-Fluorouracil (5-FU).

To observe supplemented Inula-Ochrae Decoction (SIOD) in preventing and treating nausea and vomiting induced by chemotherapy for patients with malignant tumour.

To study the effective orally taken medicine in treating the leukopenia.

To explore the risk of pregnancy in patients conceived within one year after successful chemotherapy for gestational trophoblastic tumor.

To study the effect of bhrfl expressing on the topoisomerase I expression in nasopharyngeal carcinoma cell line CNE2.

The cDNA fragment of the first 3 loops of VEGF receptor, KDR, was cloned by PCR and inserted into a baculovirus expression plasmid pFASTBACI. The competent E. coli DH10BAC cell, which contain another plasmid with baculovirus genome in it, was transformed with pFASTBACI-KDRn3. Homologous recombination in the prokaryotic cells resulted in a recombinant plasmid containing KDRn3 in baculovirus genome. Transfection of the insect cell SF-9 with above plasmid generated a recombinant baculorvirus contain target gene fragment. SDS-PAGE and Western blot analysis of the supernatant of the infected SF-9 cell showed that KDRn3 was secreted in the medium. The recombinant protein was verified with Western blot and tested for their binding activity with VEGF. Its anti-angiogenic activity was assayed on chorionic allantoic membrane(CAM) of fertilized egg. The results showed that the recombinant protein could inhibit new vessel formation on CAM of fertilized eggs.

Adriamycin-induced nephropathy (AIN) model in rats (mainlining 7.5 mg/kg avoirdupois adiramycine) was adopted to explore the effect of Shenfukang (SFK). The results showed that SFK could remarkably reduce the content of urine protein, cholesterol, creatinine and urinary nitrogen in serum, MDA in serum and renal cortex of AIN model rats. And SFK also could increase the content of album and golbulin in serum, the activities of SOD in serum and renal cortex of AIN model rats.